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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy
| Title: | Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy |
| Authors: | Hasebe, Akira Browse this author →KAKEN DB | | Ishikawa, Isao Browse this author →KAKEN DB | | Shamsul, Haque M Browse this author | | Ohtani, Makoto Browse this author | | Segawa, Taku Browse this author | | Saeki, Ayumi Browse this author | | Tanizume, Naoho Browse this author | | Oouchi, Manabu Browse this author | | Okagami, Yoshihide Browse this author | | Okano, Teruo Browse this author →KAKEN DB | | Shibata, Ken-ichiro Browse this author →KAKEN DB |
| Issue Date: | Mar-2013 |
| Publisher: | Mary Ann Liebert |
| Journal Title: | Photomedicine and Laser Surgery |
| Volume: | 31 |
| Issue: | 3 |
| Start Page: | 1 |
| End Page: | 7 |
| Publisher DOI: | 10.1089/pho.2012.3372 |
| PMID: | 23402393 |
| Abstract: | Objective: The objective of this research was to determine the effectiveness of antimicrobial photodynamic therapy (aPDT) in the removal of mycoplasmas from contaminated cells. Background data: Mycoplasmas often contaminate cell cultures. The cell-contaminating mycoplasmas are removed by antibiotics, but the use of antibiotics usually induces antibiotic-resistant bacteria. aPDT is expected to be a possible alternative to antibiotic treatments for suppressing infections. Materials and Methods: Mycoplasma salivarium (Ms)-infected human embryonic kidney (HEK) 293 cells were irradiated using a red light-emitting diode (LED) in the presence of methylene blue (MB) as a photosensitizer. The Ms viable count was determined using culture on agar plates or using a mycoplasma detection kit. Results: aPDT performed using red LED irradiation was effective in decreasing live Ms in the presence of MB without damaging the HEK293 cells. aPDT removed live Ms from the infected cells after washing the cells with sterilized phosphate-buffered saline (PBS) to decrease the initial number of live Ms before aPDT. Conclusions: This study suggests that aPDT could remove mycoplasmas from contaminated cells. |
| Rights: | This is a copy of an article published in the Photomedicine and Laser Surgery © 2013 copyright Mary Ann Liebert, Inc.; Photomedicine and Laser Surgery is available online at: http://online.liebertpub.com. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/54727 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 長谷部 晃
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