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Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy

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Title: Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy
Authors: Hasebe, Akira Browse this author →KAKEN DB
Ishikawa, Isao Browse this author →KAKEN DB
Shamsul, Haque M Browse this author
Ohtani, Makoto Browse this author
Segawa, Taku Browse this author
Saeki, Ayumi Browse this author
Tanizume, Naoho Browse this author
Oouchi, Manabu Browse this author
Okagami, Yoshihide Browse this author
Okano, Teruo Browse this author →KAKEN DB
Shibata, Ken-ichiro Browse this author →KAKEN DB
Issue Date: Mar-2013
Publisher: Mary Ann Liebert
Journal Title: Photomedicine and Laser Surgery
Volume: 31
Issue: 3
Start Page: 1
End Page: 7
Publisher DOI: 10.1089/pho.2012.3372
PMID: 23402393
Abstract: Objective: The objective of this research was to determine the effectiveness of antimicrobial photodynamic therapy (aPDT) in the removal of mycoplasmas from contaminated cells. Background data: Mycoplasmas often contaminate cell cultures. The cell-contaminating mycoplasmas are removed by antibiotics, but the use of antibiotics usually induces antibiotic-resistant bacteria. aPDT is expected to be a possible alternative to antibiotic treatments for suppressing infections. Materials and Methods: Mycoplasma salivarium (Ms)-infected human embryonic kidney (HEK) 293 cells were irradiated using a red light-emitting diode (LED) in the presence of methylene blue (MB) as a photosensitizer. The Ms viable count was determined using culture on agar plates or using a mycoplasma detection kit. Results: aPDT performed using red LED irradiation was effective in decreasing live Ms in the presence of MB without damaging the HEK293 cells. aPDT removed live Ms from the infected cells after washing the cells with sterilized phosphate-buffered saline (PBS) to decrease the initial number of live Ms before aPDT. Conclusions: This study suggests that aPDT could remove mycoplasmas from contaminated cells.
Rights: This is a copy of an article published in the Photomedicine and Laser Surgery © 2013 copyright Mary Ann Liebert, Inc.; Photomedicine and Laser Surgery is available online at:
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷部 晃

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