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Lysosomal Interaction of Akt with Phafin2 : A Critical Step in the Induction of Autophagy
This item is licensed under:Creative Commons Attribution 3.0 Unported
| Title: | Lysosomal Interaction of Akt with Phafin2 : A Critical Step in the Induction of Autophagy |
| Authors: | Matsuda-Lennikov, Mami Browse this author | | Suizu, Futoshi Browse this author →KAKEN DB | | Hirata, Noriyuki Browse this author | | Hashimoto, Manabu Browse this author | | Kimura, Kohki Browse this author | | Nagamine, Tadashi Browse this author | | Fujioka, Yoichiro Browse this author | | Ohba, Yusuke Browse this author →KAKEN DB | | Iwanaga, Toshihiko Browse this author →KAKEN DB | | Noguchi, Masayuki Browse this author →KAKEN DB |
| Issue Date: | 8-Jan-2014 |
| Publisher: | Public library science |
| Journal Title: | Plos one |
| Volume: | 9 |
| Issue: | 1 |
| Start Page: | e79795 |
| Publisher DOI: | 10.1371/journal.pone.0079795 |
| PMID: | 24416124 |
| Abstract: | Autophagy is an evolutionarily conserved mechanism for the gross disposal of intracellular proteins in mammalian cells and dysfunction in this pathway has been associated with human disease. Although the serine threonine kinase Akt is suggested to play a role in this process, little is known about the molecular mechanisms by which Akt induces autophagy. Using a yeast two-hybrid screen, Phafin2 (EAPF or PLEKHF2), a lysosomal protein with a unique structure of N-terminal PH (pleckstrin homology) domain and C-terminal FYVE (Fab 1, YOTB, Vac 1, and EEA1) domain was found to interact with Akt. A sucrose gradient fractionation experiment revealed that both Akt and Phafin2 co-existed in the same lysosome enriched fraction after autophagy induction. Confocal microscopic analysis and BiFC analysis demonstrated that both Akt and Phafin2 accumulate in the lysosome after induction of autophagy. BiFC analysis using PtdIns (3) P interaction defective mutant of Phafin2 demonstrated that lysosomal accumulation of the Akt-Phafin2 complex and subsequent induction of autophagy were lysosomal PtdIns (3) P dependent events. Furthermore, in murine macrophages, both Akt and Phafin2 were required for digestion of fluorescent bacteria and/or LPS-induced autophagy. Taken together, these findings establish that lysosomal accumulation of Akt and Phafin2 is a critical step in the induction of autophagy via an interaction with PtdIns (3)P. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/54739 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 野口 昌幸
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