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Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction

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Title: Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction
Authors: Kurasaki, Masaaki Browse this author →KAKEN DB
Sun, Yongkun Browse this author
Komori, Miyako Browse this author
Miyajima, Miki Browse this author
Hosokawa, Toshiyuki Browse this author →KAKEN DB
Saito, Takeshi Browse this author →KAKEN DB
Keywords: DNA Damage
Quantification
TUNEL
Apoptosis
PC12 Cells
Issue Date: Aug-2012
Publisher: Scientific Research Publishing Inc.
Journal Title: Advances in Biological Chemistry
Volume: 2
Issue: 3
Start Page: 243
End Page: 247
Publisher DOI: 10.4236/abc.2012.23030
Abstract: An improved terminal deoxynucleotidyl transferase- mediated dUTP-biotin nick end labeling method for the quantification of DNA damage in tissues and cultured cells was developed. Many reports have revealed that histochemistry of DNA damage can be visualized using immunohistochemistry for the terminal deoxynucleotidyl transferase reaction in tissue sections. However, few reports have described quantification of DNA damage in tissues or cells. In this study, to estimate the degree of DNA damage, the confirmed method for histochemistry using biotinylated dUTP and deoxynucleotidyl transferase was applied to label the cleaved DNA ends caused by DNA damage in tissues or cells. After end-labeling, avidin-conjugated peroxidase was reacted. A significant correlation was observed between numbers of cleaved DNA ends and peroxidase activity after the reaction. The obtained signals for presented method showed higher than those for ordinary method, and correlate with degree of DNA damage caused by serum deprivation and chemical dose. In addition, DNA damage caused by apoptosis in cells treated with 6-hydroxydopamine or Cu and in the tissues of rats administered a diet containing no Zn could be evaluated quantitatively using the present method.
Rights: Copyright © 2012 by authors and Scientific Research Publishing Inc.
http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/54753
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藏崎 正明

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