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Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction
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| Title: | Measurement of DNA damage by terminal deoxynucleotidyl transferase reaction |
| Authors: | Kurasaki, Masaaki Browse this author →KAKEN DB | | Sun, Yongkun Browse this author | | Komori, Miyako Browse this author | | Miyajima, Miki Browse this author | | Hosokawa, Toshiyuki Browse this author →KAKEN DB | | Saito, Takeshi Browse this author →KAKEN DB |
| Keywords: | DNA Damage | | Quantification | | TUNEL | | Apoptosis | | PC12 Cells |
| Issue Date: | Aug-2012 |
| Publisher: | Scientific Research Publishing Inc. |
| Journal Title: | Advances in Biological Chemistry |
| Volume: | 2 |
| Issue: | 3 |
| Start Page: | 243 |
| End Page: | 247 |
| Publisher DOI: | 10.4236/abc.2012.23030 |
| Abstract: | An improved terminal deoxynucleotidyl transferase- mediated dUTP-biotin nick end labeling method for the quantification of DNA damage in tissues and cultured cells was developed. Many reports have revealed that histochemistry of DNA damage can be visualized using immunohistochemistry for the terminal deoxynucleotidyl transferase reaction in tissue sections. However, few reports have described quantification of DNA damage in tissues or cells. In this study, to estimate the degree of DNA damage, the confirmed method for histochemistry using biotinylated dUTP and deoxynucleotidyl transferase was applied to label the cleaved DNA ends caused by DNA damage in tissues or cells. After end-labeling, avidin-conjugated peroxidase was reacted. A significant correlation was observed between numbers of cleaved DNA ends and peroxidase activity after the reaction. The obtained signals for presented method showed higher than those for ordinary method, and correlate with degree of DNA damage caused by serum deprivation and chemical dose. In addition, DNA damage caused by apoptosis in cells treated with 6-hydroxydopamine or Cu and in the tissues of rats administered a diet containing no Zn could be evaluated quantitatively using the present method. |
| Rights: | Copyright © 2012 by authors and Scientific Research Publishing Inc. | | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/54753 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 藏崎 正明
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