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Androgenetic doubled haploids induced without irradiation of eggs in loach (Misgurnus anguillicaudatus)

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Title: Androgenetic doubled haploids induced without irradiation of eggs in loach (Misgurnus anguillicaudatus)
Authors: Hou, Jilun Browse this author
Saito, Taiju Browse this author
Fujimoto, Takafumi Browse this author
Yamaha, Etsuro Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Keywords: Chromosome manipulation
Inbred line
Issue Date: 20-Jan-2014
Publisher: Elsevier
Journal Title: Aquaculture
Volume: 420-421
Issue: Supplement 1
Start Page: S57
End Page: S63
Publisher DOI: 10.1016/j.aquaculture.2013.05.021
Abstract: Androgenetic doubled haploids (DHs) were induced in the loach Misgurnus anguillicaudatus (Cobitidae) without irradiation of the eggs. The eggs of wild-type females were activated with the intact sperm of an orange-phenotype male, and treated (within 10 s of activation) at 3 ± 0.5 °C for 30 min, to eliminate the female nucleus. The eggs were then incubated in a water bath at 20 ± 0.5 °C for 35 min. Finally, diploidy was restored (65 min after activation) by heat-shock treatment at 42 ± 0.5 °C for 2 min. Under these conditions, the yield rate (mean ± SD) of putative DHs relative to the total number of eggs used was 10.43 ± 1.69%, which was significantly higher (P < 0.05) than the yield rates obtained under the remaining heat-shock initiation conditions (55 min, 60 min, and 70 min after activation). We analyzed the ploidy status of the putative DH by using flow cytometry. All-male inheritance was confirmed by the expression of the recessive orange body color trait and microsatellite genotypes. We detected no maternally derived alleles or heterozygous genotypes at any of the 28 loci (covering 27 linkage groups) of loach, indicating the exclusively paternal inheritance and homozygosity of the obtained androgenetic DHs.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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