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Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein

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Title: Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein
Authors: Changula, Katendi Browse this author
Yoshida, Reiko Browse this author
Noyori, Osamu Browse this author
Marzi, Andrea Browse this author
Miyamoto, Hiroko Browse this author
Ishijima, Mari Browse this author
Yokoyama, Ayaka Browse this author
Kajihara, Masahiro Browse this author
Feldmann, Heinz Browse this author
Mweene, Aaron S. Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: Ebola virus
Antibody epitope
Monoclonal antibody
Synthetic peptide
Issue Date: Sep-2013
Publisher: Elsevier
Journal Title: Virus Research
Volume: 176
Issue: 1-2
Start Page: 83
End Page: 90
Publisher DOI: 10.1016/j.virusres.2013.05.004
Abstract: Filoviruses (viruses in the genus Ebolavirus and Marburgvirus in the family Filoviridae) cause severe haemorrhagic fever in humans and nonhuman primates. Rapid, highly sensitive, and reliable filovirus-specific assays are required for diagnostics and outbreak control. Characterisation of antigenic sites in viral proteins can aid in the development of viral antigen detection assays such immunochromatography-based rapid diagnosis. We generated a panel of mouse monoclonal antibodies (mAbs) to the nucleoprotein (NP) of Ebola virus belonging to the species Zaire ebolavirus. The mAbs were divided into seven groups based on the profiles of their specificity and cross-reactivity to other species in the Ebolavirus genus. Using synthetic peptides corresponding to the Ebola virus NP sequence, the mAb binding sites were mapped to seven antigenic regions in the C-terminal half of the NP, including two highly conserved regions among all five Ebolavirus species currently known. Furthermore, we successfully produced species-specific rabbit antisera to synthetic peptides predicted to represent unique filovirus B-cell epitopes. Our data provide useful information for the development of Ebola virus antigen detection assays. (C) 2013 Elsevier B.V. All rights reserved.
Type: article (author version)
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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