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Amoebal Endosymbiont Neochlamydia Genome Sequence Illuminates the Bacterial Role in the Defense of the Host Amoebae against Legionella pneumophila

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Title: Amoebal Endosymbiont Neochlamydia Genome Sequence Illuminates the Bacterial Role in the Defense of the Host Amoebae against Legionella pneumophila
Authors: Ishida, Kasumi Browse this author
Sekizuka, Tsuyoshi Browse this author
Hayashida, Kyoko Browse this author
Matsuo, Junji Browse this author →KAKEN DB
Takeuchi, Fumihiko Browse this author
Kuroda, Makoto Browse this author
Nakamura, Shinji Browse this author →KAKEN DB
Yamazaki, Tomohiro Browse this author
Yoshida, Mitsutaka Browse this author
Takahashi, Kaori Browse this author
Nagai, Hiroki Browse this author
Sugimoto, Chihiro Browse this author →KAKEN DB
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Issue Date: 18-Apr-2014
Publisher: The Public Library of Science
Journal Title: PLoS ONE
Volume: 9
Issue: 4
Start Page: e95166
Publisher DOI: 10.1371/journal.pone.0095166
Abstract: Previous work has shown that the obligate intracellular amoebal endosymbiont Neochlamydia S13, an environmental chlamydia strain, has an amoebal infection rate of 100%, but does not cause amoebal lysis and lacks transferability to other host amoebae. The underlying mechanism for these observations remains unknown. In this study, we found that the host amoeba could completely evade Legionella infection. The draft genome sequence of Neochlamydia S13 revealed several defects in essential metabolic pathways, as well as unique molecules with leucine-rich repeats (LRRs) and ankyrin domains, responsible for protein-protein interaction. Neochlamydia S13 lacked an intact tricarboxylic acid cycle and had an incomplete respiratory chain. ADP/ATP translocases, ATP-binding cassette transporters, and secretion systems (types II and III) were well conserved, but no type IV secretion system was found. The number of outer membrane proteins (OmcB, PomS, 76-kDa protein, and OmpW) was limited. Interestingly, genes predicting unique proteins with LRRs (30 genes) or ankyrin domains (one gene) were identified. Furthermore, 33 transposases were found, possibly explaining the drastic genome modification. Taken together, the genomic features of Neochlamydia S13 explain the intimate interaction with the host amoeba to compensate for bacterial metabolic defects, and illuminate the role of the endosymbiont in the defense of the host amoebae against Legionella infection.
Type: article
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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