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Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrPSc during early stage prion infection in Neuro2a cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/55280

Title: Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrPSc during early stage prion infection in Neuro2a cells
Authors: Yamasaki, Takeshi Browse this author
Baron, Gerald S. Browse this author
Suzuki, Akio Browse this author
Hasebe, Rie Browse this author →KAKEN DB
Horiuchi, Motohiro Browse this author →KAKEN DB
Keywords: Prions
Membrane trafficking
Endosomes
Neurodegenerative diseases
Rab proteins
Issue Date: Feb-2014
Publisher: Elsevier
Journal Title: Virology
Volume: 450-451
Start Page: 324
End Page: 335
Publisher DOI: 10.1016/j.virol.2013.11.007
Abstract: To clarify the cellular mechanisms for the establishment of prion infection, we analyzed the intracellular dynamics of inoculated and newly generated abnormal isoform of prion protein (PrPSc) in Neuro2a cells. Within 24 h after inoculation, the newly generated PrPSc was evident at the plasma membrane, in early endosomes, and in late endosomes, but this PrPSc was barely evident in lysosomes; in contrast, the majority of the inoculated PrPSc was evident in late endosomes and lysosomes. However, during the subsequent 48 h, the newly generated PrPSc increased remarkably in early endosomes and recycling endosomes. Overexpression of wild-type and mutant Rab proteins showed that membrane trafficking along not only the endocytic-recycling pathway but also the endo-lysosomal pathway is involved in de novo PrPSc generation. These results suggest that the trafficking of exogenously introduced PrPSc from the endo-lysosomal pathway to the endocytic-recycling pathway is important for the establishment of prion infection. (C) 2013 Elsevier Inc. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/55280
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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