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Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrPSc during early stage prion infection in Neuro2a cells
Title: | Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrPSc during early stage prion infection in Neuro2a cells |
Authors: | Yamasaki, Takeshi Browse this author | Baron, Gerald S. Browse this author | Suzuki, Akio Browse this author | Hasebe, Rie Browse this author →KAKEN DB | Horiuchi, Motohiro Browse this author →KAKEN DB |
Keywords: | Prions | Membrane trafficking | Endosomes | Neurodegenerative diseases | Rab proteins |
Issue Date: | Feb-2014 |
Publisher: | Elsevier |
Journal Title: | Virology |
Volume: | 450-451 |
Start Page: | 324 |
End Page: | 335 |
Publisher DOI: | 10.1016/j.virol.2013.11.007 |
Abstract: | To clarify the cellular mechanisms for the establishment of prion infection, we analyzed the intracellular dynamics of inoculated and newly generated abnormal isoform of prion protein (PrPSc) in Neuro2a cells. Within 24 h after inoculation, the newly generated PrPSc was evident at the plasma membrane, in early endosomes, and in late endosomes, but this PrPSc was barely evident in lysosomes; in contrast, the majority of the inoculated PrPSc was evident in late endosomes and lysosomes. However, during the subsequent 48 h, the newly generated PrPSc increased remarkably in early endosomes and recycling endosomes. Overexpression of wild-type and mutant Rab proteins showed that membrane trafficking along not only the endocytic-recycling pathway but also the endo-lysosomal pathway is involved in de novo PrPSc generation. These results suggest that the trafficking of exogenously introduced PrPSc from the endo-lysosomal pathway to the endocytic-recycling pathway is important for the establishment of prion infection. (C) 2013 Elsevier Inc. All rights reserved. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/55280 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 堀内 基広
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