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Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome in rats

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Title: Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome in rats
Authors: Lennikov, Anton Browse this author
Kitaichi, Nobuyoshi Browse this author →KAKEN DB
Noda, Kousuke Browse this author →KAKEN DB
Mizuuchi, Kazuomi Browse this author →KAKEN DB
Ando, Ryo Browse this author →KAKEN DB
Dong, Zhenyu Browse this author →KAKEN DB
Fukuhara, Junichi Browse this author
Kinoshita, Satoshi Browse this author
Namba, Kenichi Browse this author →KAKEN DB
Ohno, Shigeaki Browse this author →KAKEN DB
Ishida, Susumu Browse this author →KAKEN DB
Issue Date: 7-Feb-2014
Publisher: Molecular Vision
Journal Title: Molecular Vision
Volume: 20
Start Page: 171
End Page: 177
PMID: 24520186
Abstract: Purpose: Echinochrome is a pigment present in the shells and spines of sea urchins. It has been reported to have several biologic protective effects, including in experimental models of myocardial ischemia/reperfusion injury, for which the proposed mechanisms are scavenging reactive oxygen species (ROS) and chelating iron. Endotoxin-induced uveitis (EIU) is an animal model of acute anterior segment intraocular inflammation that is induced by the injection of lipopolysaccharide (LPS). In this study, the therapeutic effect of echinochrome was examined in uveitis using the EIU model. Methods: EIU was induced in Lewis rats via 200 mu g subcutaneous injections of LPS from Escherichia coli. Echinochrome was administered intravenously in 10, 1, or 0.1 mg/kg doses suspended in PBS (controls were injected with PBS only). Twenty-four hours after LPS injection, the number of infiltrating cells and the protein concentration in aqueous humor were determined. Aqueous tumor necrosis factor alpha (TNF-alpha) concentration was quantified with enzyme-linked immunosorbent assay, eyes were stained with nuclear factor (NF) kappa B antibodies, and ROS production was determined by dihydroethidium staining in fresh frozen samples. Results: The number of inflammatory aqueous cells and protein levels were lower in the groups treated with 10 and 1 mg/kg of echinochrome than in the untreated LPS group (p<0.01). Treatment with 10 and 1 mg/kg of echinochrome significantly reduced TNF-alpha concentrations in aqueous humor (p<0.01). The numbers of NF kappa B-positive cells and ROS signals were also reduced by echinochrome administration (p<0.05). Conclusions: Echinochrome ameliorated intraocular inflammation caused by EIU by reducing ROS production, thereby also decreasing the expression of NF kappa B and TNF-alpha. As a natural pigment, echinochrome may therefore be a promising candidate for the safe treatment of intraocular inflammation. The use of sea urchin shells and spines in health foods and medical products is thus both economically and environmentally meaningful.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石田 晋

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