HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube

Creative Commons License

Files in This Item:
PLoS One_9(2)_e89400.pdf957.37 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/55349

Title: The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube
Authors: Horne, Andrew W. Browse this author
Brown, Jeremy K. Browse this author
Nio-Kobayashi, Junko Browse this author →KAKEN DB
Abidin, Hazirah B. Z. Browse this author
Adin, Zety E. H. A. Browse this author
Boswell, Lyndsey Browse this author
Burgess, Stewart Browse this author
Lee, Kai-Fai Browse this author
Duncan, W. Colin Browse this author
Issue Date: 20-Feb-2014
Publisher: PLOS
Journal Title: Plos One
Volume: 9
Issue: 2
Start Page: e89400
Publisher DOI: 10.1371/journal.pone.0089400
PMID: 24586750
Abstract: Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/ E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P, 0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P<0.01) and increased levels of BCL2 transcript (P<0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P<0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy.
Rights: http://creativecommons.org/licenses/by/3.0/
Type: article
URI: http://hdl.handle.net/2115/55349
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 純子

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University