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Hokkaido University Collection of Scholarly and Academic Papers >
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The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube
This item is licensed under:Creative Commons Attribution 3.0 Unported
| Title: | The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube |
| Authors: | Horne, Andrew W. Browse this author | | Brown, Jeremy K. Browse this author | | Nio-Kobayashi, Junko Browse this author →KAKEN DB | | Abidin, Hazirah B. Z. Browse this author | | Adin, Zety E. H. A. Browse this author | | Boswell, Lyndsey Browse this author | | Burgess, Stewart Browse this author | | Lee, Kai-Fai Browse this author | | Duncan, W. Colin Browse this author |
| Issue Date: | 20-Feb-2014 |
| Publisher: | PLOS |
| Journal Title: | Plos One |
| Volume: | 9 |
| Issue: | 2 |
| Start Page: | e89400 |
| Publisher DOI: | 10.1371/journal.pone.0089400 |
| PMID: | 24586750 |
| Abstract: | Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/ E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P, 0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P<0.01) and increased levels of BCL2 transcript (P<0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P<0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/55349 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小林 純子
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