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Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain
| Title: | Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain |
| Authors: | Asano, Nozomi Browse this author | | Atsuumi, Haruka Browse this author | | Nakamura, Akiyoshi Browse this author | | Tanaka, Yoshikazu Browse this author | | Tanaka, Isao Browse this author →KAKEN DB | | Yao, Min Browse this author →KAKEN DB |
| Keywords: | Ribosome biogenesis | | Interaction | | Intrinsically disordered protein | | Isothermal titration calorimetty | | Circular dichroism |
| Issue Date: | 24-Jan-2014 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 443 |
| Issue: | 4 |
| Start Page: | 1251 |
| End Page: | 1256 |
| Publisher DOI: | 10.1016/j.bbrc.2013.12.143 |
| PMID: | 24406167 |
| Abstract: | Removal of anti-association factor, Tif6 (eIF6), by elongation factor-like 1 (EFL1) and Shwachman-Bodian-Diamond syndrome (SBDS) protein is a critical step in the late stage of ribosome maturation. Although EFL1 is known to have GTPase activity that is stimulated by SBDS, how they cooperatively trigger dissociation of Tif6 from the ribosome remains to be elucidated. In the present study, the interaction between EFL1 and SBDS was analyzed by size exclusion chromatography, gel shift assay, and isothermal titration calorimetry (ITC). The results showed that EFL1 interacted directly with SBDS. ITC experiments using domain-truncated mutants showed that the interaction between EFL1 and SBDS is governed by the insertion domain of EFL1 and domains II-III of SBDS. Circular dichroism spectroscopy showed that the insertion domain of EFL1 has a random structure in the absence of SBDS, whereas the disadvantageous entropy change observed on ITC suggested a fixed conformation coupled with complex formation with SBDS. Based on these observations together with those reported previously, we propose roles of EFL1 and SBDS in ribosomal maturation. (C) 2014 Elsevier Inc. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/55598 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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