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Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/55598

Title: Direct interaction between EFL1 and SBDS is mediated by an intrinsically disordered insertion domain
Authors: Asano, Nozomi Browse this author
Atsuumi, Haruka Browse this author
Nakamura, Akiyoshi Browse this author
Tanaka, Yoshikazu Browse this author
Tanaka, Isao Browse this author →KAKEN DB
Yao, Min Browse this author →KAKEN DB
Keywords: Ribosome biogenesis
Interaction
Intrinsically disordered protein
Isothermal titration calorimetty
Circular dichroism
Issue Date: 24-Jan-2014
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 443
Issue: 4
Start Page: 1251
End Page: 1256
Publisher DOI: 10.1016/j.bbrc.2013.12.143
PMID: 24406167
Abstract: Removal of anti-association factor, Tif6 (eIF6), by elongation factor-like 1 (EFL1) and Shwachman-Bodian-Diamond syndrome (SBDS) protein is a critical step in the late stage of ribosome maturation. Although EFL1 is known to have GTPase activity that is stimulated by SBDS, how they cooperatively trigger dissociation of Tif6 from the ribosome remains to be elucidated. In the present study, the interaction between EFL1 and SBDS was analyzed by size exclusion chromatography, gel shift assay, and isothermal titration calorimetry (ITC). The results showed that EFL1 interacted directly with SBDS. ITC experiments using domain-truncated mutants showed that the interaction between EFL1 and SBDS is governed by the insertion domain of EFL1 and domains II-III of SBDS. Circular dichroism spectroscopy showed that the insertion domain of EFL1 has a random structure in the absence of SBDS, whereas the disadvantageous entropy change observed on ITC suggested a fixed conformation coupled with complex formation with SBDS. Based on these observations together with those reported previously, we propose roles of EFL1 and SBDS in ribosomal maturation. (C) 2014 Elsevier Inc. All rights reserved.
Type: article (author version)
URI: http://hdl.handle.net/2115/55598
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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