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Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion

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タイトル: Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion
著者: Yoshii, Kentarou 著作を一覧する
Konno, Akihiro 著作を一覧する
Goto, Akiko 著作を一覧する
Nio, Junko 著作を一覧する
Obara, Mayumi 著作を一覧する
Ueki, Tomotaka 著作を一覧する
Hayasaka, Daisuke 著作を一覧する
Mizutani, Tetsuya 著作を一覧する
Kariwa, Hiroaki 著作を一覧する
Takashima, Ikuo 著作を一覧する
発行日: 2004年
出版者: Society for General Microbiology
誌名: Journal of General Virology
巻: 85
開始ページ: 3049
終了ページ: 3058
出版社 DOI: 10.1099/vir.0.80169-0
抄録: Flaviviruses are assembled to bud into the lumen of the endoplasmic reticulum (ER) and are secreted through the vesicle transport pathway. Virus envelope proteins play important roles in this process. In this study, the effect of mutations in the envelope proteins of tick-borne encephalitis (TBE) virus on secretion of virus-like particles (VLPs), using a recombinant plasmid expression system was analysed. It was found that a single point mutation at position 63 in prM induces a reduction in secretion of VLPs. The mutation in prM did not affect the folding of the envelope proteins, and chaperone-like activity of prM was maintained. As observed by immunofluorescence microscopy, viral envelope proteins with the mutation in prM were scarce in the Golgi complex, and accumulated in the ER. Electron microscopic analysis of cells expressing the mutated prM revealed that many tubular structures were present in the lumen. The insertion of the prM mutation at aa 63 into the viral genome reduced the production of infectious virus particles. This data suggest that prM plays a crucial role in the virus budding process.
資料タイプ: article
URI: http://hdl.handle.net/2115/5570
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高島 郁夫

 

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