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Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion

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Title: Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion
Authors: Yoshii, Kentarou Browse this author →KAKEN DB
Konno, Akihiro Browse this author →KAKEN DB
Goto, Akiko Browse this author
Nio, Junko Browse this author
Obara, Mayumi Browse this author
Ueki, Tomotaka Browse this author
Hayasaka, Daisuke Browse this author
Mizutani, Tetsuya Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Takashima, Ikuo10 Browse this author →KAKEN DB
Authors(alt): 高島, 郁夫10
Issue Date: 2004
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 85
Start Page: 3049
End Page: 3058
Publisher DOI: 10.1099/vir.0.80169-0
PMID: 15448368
Abstract: Flaviviruses are assembled to bud into the lumen of the endoplasmic reticulum (ER) and are secreted through the vesicle transport pathway. Virus envelope proteins play important roles in this process. In this study, the effect of mutations in the envelope proteins of tick-borne encephalitis (TBE) virus on secretion of virus-like particles (VLPs), using a recombinant plasmid expression system was analysed. It was found that a single point mutation at position 63 in prM induces a reduction in secretion of VLPs. The mutation in prM did not affect the folding of the envelope proteins, and chaperone-like activity of prM was maintained. As observed by immunofluorescence microscopy, viral envelope proteins with the mutation in prM were scarce in the Golgi complex, and accumulated in the ER. Electron microscopic analysis of cells expressing the mutated prM revealed that many tubular structures were present in the lumen. The insertion of the prM mutation at aa 63 into the viral genome reduced the production of infectious virus particles. This data suggest that prM plays a crucial role in the virus budding process.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高島 郁夫

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