|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
A Ca2+-dependent signalling circuit regulates influenza A virus internalization and infection
| Title: | A Ca2+-dependent signalling circuit regulates influenza A virus internalization and infection |
| Authors: | Fujioka, Yoichiro Browse this author | | Tsuda, Masumi Browse this author →KAKEN DB | | Nanbo, Asuka Browse this author →KAKEN DB | | Hattori, Tomoe Browse this author | | Sasaki, Junko Browse this author | | Sasaki, Takehiko Browse this author | | Miyazaki, Tadaaki Browse this author →KAKEN DB | | Ohba, Yusuke Browse this author →KAKEN DB |
| Issue Date: | 14-Nov-2013 |
| Publisher: | Nature publishing group |
| Journal Title: | Nature communications |
| Volume: | 4 |
| Start Page: | 2763 |
| Publisher DOI: | 10.1038/ncomms3763 |
| PMID: | 24434940 |
| Abstract: | Various viruses enter host cells via endocytosis, but the molecular mechanisms underlying the specific internalization pathways remain unclear. Here we show that influenza A viruses (IAVs) enter cells via redundant pathways of clathrin-mediated and clathrin-independent endocytosis, with intracellular Ca2+ having a central role in regulation of both pathways by activating a signalling axis comprising RhoA, Rho-kinase, phosphatidylinositol 4-phosphate 5-kinase (PIP5K) and phospholipase C (PLC). IAV infection induces oscillations in the cytosolic Ca2+ concentration of host cells, the prevention of which markedly attenuates virus internalization and infection. The small GTPase RhoA is found both to function downstream of the virus-induced Ca2+ response and itself to induce Ca2+ oscillations in a manner dependent on Rho-kinase and subsequent PIP5K-PLC signalling. This signalling circuit regulates both clathrin-mediated and clathrin-independent endocytosis during virus infection and seems to constitute a key mechanism for regulation of IAV internalization and infection. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/55820 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 大場 雄介
|
