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がん細胞におけるミトコンドリア由来活性酸素を介した3-Methyl pyruvateによる放射線感受性の増強

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Title: がん細胞におけるミトコンドリア由来活性酸素を介した3-Methyl pyruvateによる放射線感受性の増強
Other Titles: 3-Methyl pyruvate enhances the radiosensitivity through increase of mitochondria derivedreactive oxygen species in tumor cell lines
Authors: 西田, 直哉1 Browse this author
Authors(alt): Nishida, Naoya1
Issue Date: 25-Mar-2014
Abstract: Considerable interest has recently been focused on the special characteristics of cancer metabolism, and several drugs designed to modulate cancer metabolism have been tested as potential anticancer agents. To date, however, very few studies have been conducted to investigate the combined effects of anticancer drugs and radiotherapy. In this study, to evaluate the role of mitochondria-derived reactive oxygen species (ROS) in the radiation-induced cell death of tumor cells, we have examined the effect of 3-methyl pyruvate (MP). MP is a membrane-permeable pyruvate derivative that is capable of activating mitochondrial energy metabolism, on human lung carcinoma A549 cells and murine squamous carcinoma SCCVII cells. Pretreatment with MP significantly enhanced radiation-induced cell death in both cell lines, and also led to increases in themitochondrial membrane potential, intracellular adenosine triphosphate content, and mitochondria-derived ROS production following the exposure of the cells to X-rays. In A549 cells, MP-induced radiosensitization was completely abolished by vitamin C. In contrast, it was partially abolished in SCCVII cells. These results therefore suggest that the treatment of the cells with MP induced radiosensitization via the production of excess mitochondria-derived ROS in tumor cells.
Conffering University: 北海道大学
Degree Report Number: 甲第11283号
Degree Level: 博士
Degree Discipline: 獣医学
Examination Committee Members: (主査) 教授 稲波 修, 教授 石塚 真由美, 准教授 佐藤 耕太, 准教授 山盛 徹
Degree Affiliation: 獣医学研究科(獣医学専攻)
Type: theses (doctoral)
Appears in Collections:学位論文 (Theses) > 博士 (獣医学)
課程博士 (Doctorate by way of Advanced Course) > 獣医学研究科(Graduate School of Veterinary Medicine)

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