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成長期におけるソフトフード摂取がラット顎関節に与える影響 [全文の要約]
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| Title: | 成長期におけるソフトフード摂取がラット顎関節に与える影響 [全文の要約] |
| Authors: | 加藤, 剛士 Browse this author |
| Keywords: | 液状食 | | 顎関節 | | 成長期 | | 組織計量 | | BrdU |
| Issue Date: | 25-Mar-2014 |
| Publisher: | Hokkaido University |
| Abstract: | Modern people tends to have soft food . Therefore experimental studies have been performed to examine unfavorable influences to growth of masticatory muscles and craniofacial bone induced by such a dietary habits. The aim of the present study was to clarify the effects of soft diet on the temporomandibular joint (TMJ) in growing rats using the histomorphometrical and immunohistochemical methods. Twenty-four male Wistar rats were weaned at age 21 days and were divided into control and experimental groups. Control rats were fed a solid food and experimental ones fed a liquid food from 1 to 8 weeks. After injection with 5-bromo-2’-deoxyuridine (BrdU), the animals were perfused with 4% paraformaldehyde solution and the whole heads were removed. Serial coronal sections of TMJ were stained with Haematoxylin and Eosinor with 27 BrdU-immunohistochemistry. Three dimensions and the thickness of cartilage layer of the TMJ were measured using histological sections, and cell proliferation in the TMJ was examined using immunostained sections. The height and width of the zygomatic process of mandibular fossa in experimental group were smaller than in control group after 4 weeks. The width and length of the condyle of experimental animals were also smaller than those of controls after 4 weeks. In the mandibular fossa, articular zone (AZ) and hypertrophic zone (HZ) at 4 weeks and AZ and intermediate zone (IZ) at 8 weeks in the experimental groups were thinner than in the control. All zones at 4 weeks and AZ at 8 weeks of the condyle in the experimental group were also thinner. The labeling indices of BrdU in IZ of the mandibular fossa and of the condyle in the experimantal groups were lower than in the controls at 4 weeks and at 1 and 4 weeks, respectively. These findings suggest that the soft food-intake inhibits the growth of the TMJ of rats, due to low proliferative activity of cells in IZ. | | 最近では軟らかい食事を好んで摂取している現代人が増加している.このような食習慣が咀嚼筋や顔面骨格に与える影響を調べるため,実験的研究が行われてきた.本研究では,成長期ラットにソフトフードを継続的に摂取させた時,顎関節にどのような影響が及ぶのかを形態学的,免疫組織化学的に明らかにすることを目的とした.実験にはWistar系雄性ラット24匹を用い, 21日齢で離乳させた.離乳後,液状食（実験群）あるいは固形食（対照群）を与え, 1～8週間飼育した.飼育期間が終了した動物は5-bromo-2’-deoxyuridine（BrdU）を投与した後,4%パラホルムアルデヒド溶液にて灌流固定し,頭部を摘出した.以後通法に従って前頭断連続切片を作製した.切片にはヘマトキシリン・エオジン染色を施し,顎関節部の3次元的大きさ,および軟骨層の厚さについて組織計量を行った.また一部の切片にはBrdU免疫染色を施し,顎関節部の細胞増殖活性を測定した.組織計量学的には,実験群の下顎窩相当部頬骨突起は4週（高径）と8週（幅径、高径）で,下顎頭は4週（幅径）と8週（幅径、長径）で対照群よりも小さかった.軟骨層の厚さに関しては,下顎窩では4週のarticular zone(AZ)とhypertrophic zone(HZ), 8週のAZとintermediate zone(IZ)で,下顎頭では4週の3層全てと8週のAZで実験群の方が菲薄であった.免疫組織化学的には, BrdU陽性細胞のほとんどはIZに認められた.BrdU陽性率では対照群より実験群が概して低く,下顎窩では4週,下顎頭では1週と4週で有意差が認められた.以上の結果から成長期におけるソフトフード摂取はラット顎関節の成長を阻害することが明らかとなり,これにはIZ中の細胞増殖活性低下が関連していると考えられた. |
| Description: | この博士論文全文の閲覧方法については、以下のサイトをご参照ください。 |
| Description URI: | https://www.lib.hokudai.ac.jp/dissertations/copy-guides/ |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第11246号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 土門 卓文, 教授 鄭 漢忠, 准教授 高橋 茂, 講師 上田 康夫 |
| Degree Affiliation: | 歯学研究科（口腔医学専攻） |
| Type: | theses (doctoral - abstract of entire text) |
| URI: | http://hdl.handle.net/2115/56137 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （歯学）
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
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