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Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats.

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Title: Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats.
Other Titles: アスコルビン酸合成能欠如(ODS)ラットの骨組織石灰化における組織学的解析
Authors: 長谷川, 智香 Browse this author →KAKEN DB
Issue Date: 25-Mar-2014
Abstract: Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an ascorbic acid-deficient (aa-def) diet. As aa-def ODS rats were shown to feature disordered bone formation, we have examined the bone mineralization in this rat model. A fibrous tissue layer surrounding the trabeculae of tibial metaphyses was found in aa-def ODS rats, and this layer showed intense alkaline phosphatase activity and proliferating cell nuclear antigen-immunopositivity. Many osteoblasts detached from the bone surfaces and were characterized by round-shaped rough endoplasmic reticulum (rER), suggesting accumulation of malformed collagen inside the rER. Accordingly, fine, fragile fibrillar collagenous structures without evident striation were found in aa-def bones, which may result from misassembling of the triple helices of collagenous α-chains. Despite a marked reduction in bone formation, ascorbic acid deprivation seemed to have no effect on mineralization : while reduced in number, normal matrix vesicles and mineralized nodules could be seen in aa-def bones. Fine needle-like mineral crystals extended from the semineralized nodules, and were apparently bound to collagenous fibrillar structures. In summary, collagen mineralization seems unaffected by ascorbic acid deficiency in spite of the fine, fragile collagenous fibrils identified in the bones of our animal model.
Conffering University: 北海道大学
Degree Report Number: 甲第11260号
Degree Level: 博士
Degree Discipline: 歯学
Examination Committee Members: (主査) 教授 網塚 憲生, 教授 鈴木 邦明, 教授 土門 卓文
Degree Affiliation: 歯学研究科(口腔医学専攻)
Type: theses (doctoral)
Appears in Collections:学位論文 (Theses) > 博士 (歯学)
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)

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