|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-beta-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite
| Title: | CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-beta-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite |
| Authors: | Kitaoka, Naoki Browse this author | | Kawaide, Hiroshi Browse this author | | Amano, Naruki Browse this author | | Matsubara, Takuya Browse this author | | Nabeta, Kensuke Browse this author | | Takahashi, Kosaku Browse this author | | Matsuura, Hideyuki Browse this author |
| Keywords: | Arabidopsis thaliana | | Cruciferac | | Glycine max | | Leguminosae | | Nicotiana tabacum | | Solanaceae | | Metabolic pathway | | Jasmonate | | Cytochrome P450 | | CYP94B3 |
| Issue Date: | Mar-2014 |
| Publisher: | Elsevier |
| Journal Title: | Phytochemistry |
| Volume: | 99 |
| Start Page: | 6 |
| End Page: | 13 |
| Publisher DOI: | 10.1016/j.phytochem.2013.12.019 |
| PMID: | 24467969 |
| Abstract: | The hormonal action of jasmonate in plants is controlled by the precise balance between its biosynthesis and inactivation. Oxidation of jasmonoyl-L-isoleucine at the C-12 position, which is catalyzed by cytochrome P450s CYP94B3 and CYP94C1, is thought to be one of the main inactivation pathways. In this study, an additional function of CYP94B3 was elucidated, as well additional jasmonoyl-L-isoleucine metabolites being investigated. It was found that CYP94B3 also catalyzes the hydroxylation of jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and that these hydroxyl compounds accumulated after wounding and possessed lower activity than non-hydroxylated compounds. Additionally, 12-O-beta-glucopyranosyl-jasmonoyl-t-isoleucine accumulated after wounding, suggesting that it is a metabolite of |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/56238 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 松浦 英幸
|
