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Bone augmentation by highly porous β-TCP scaffold coated with PLGA and FGF2 [an abstract of entire text]

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Title: Bone augmentation by highly porous β-TCP scaffold coated with PLGA and FGF2 [an abstract of entire text]
Other Titles: 高気孔性PLGA /β-TCPスキャフォールドとFGF2による骨増生効果 [全文の要約]
Authors: 吉田, 崇 Browse this author
Keywords: β-tricalcium phosphate (β-TCP)
co-poly lactic acid /glycolic acid (PLGA)
fibroblast growth factor-2 (FGF2)
bone tissue engineering
regenerative scaffold
Issue Date: 25-Mar-2014
Publisher: Hokkaido University
Abstract: β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) and fibroblast growth factor-2 (FGF2) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the PLGA/FGF2-coated β-TCP scaffold on bone augmentation. The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture and a subcutaneous implant test. Subsequently, the β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffold loaded with FGF2, were implanted into rat cranial bone. Histological observations were taken at 10 and 35 days post-surgery. SEM and TEM observations showed a thin PLGA layer on the β-TCP particle after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater compared to the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples from bone implanted with PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation, suggesting that FGF2-loaded PLGA/β-TCP scaffold was expected for bone tissue engineering.
Description: この博士論文全文の閲覧方法については、以下のサイトをご参照ください。
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Conffering University: 北海道大学
Degree Report Number: 甲第11263号
Degree Level: 博士
Degree Discipline: 歯学
Examination Committee Members: (主査) 教授 川浪 雅光, 教授 田村 正人, 教授 網塚 憲生
Degree Affiliation: 歯学研究科(口腔医学専攻)
Type: theses (doctoral - abstract of entire text)
Appears in Collections:学位論文 (Theses) > 博士 (歯学)
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)

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