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Bone augmentation by highly porous β-TCP scaffold coated with PLGA and FGF2 [an abstract of entire text]
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| Title: | Bone augmentation by highly porous β-TCP scaffold coated with PLGA and FGF2 [an abstract of entire text] |
| Other Titles: | 高気孔性PLGA /β-TCPスキャフォールドとFGF2による骨増生効果 [全文の要約] |
| Authors: | 吉田, 崇 Browse this author |
| Keywords: | β-tricalcium phosphate (β-TCP) | | co-poly lactic acid /glycolic acid (PLGA) | | fibroblast growth factor-2 (FGF2) | | bone tissue engineering | | regenerative scaffold |
| Issue Date: | 25-Mar-2014 |
| Publisher: | Hokkaido University |
| Abstract: | β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) and fibroblast growth factor-2 (FGF2) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the PLGA/FGF2-coated β-TCP scaffold on bone augmentation. The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture and a subcutaneous implant test. Subsequently, the β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffold loaded with FGF2, were implanted into rat cranial bone. Histological observations were taken at 10 and 35 days post-surgery. SEM and TEM observations showed a thin PLGA layer on the β-TCP particle after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater compared to the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples from bone implanted with PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation, suggesting that FGF2-loaded PLGA/β-TCP scaffold was expected for bone tissue engineering. |
| Description: | この博士論文全文の閲覧方法については、以下のサイトをご参照ください。 |
| Description URI: | https://www.lib.hokudai.ac.jp/dissertations/copy-guides/ |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第11263号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 川浪 雅光, 教授 田村 正人, 教授 網塚 憲生 |
| Degree Affiliation: | 歯学研究科（口腔医学専攻） |
| Type: | theses (doctoral - abstract of entire text) |
| URI: | http://hdl.handle.net/2115/56241 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （歯学）
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
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