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Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins

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Title: Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins
Authors: Chidumayo, Nozyechi N. Browse this author
Yoshii, Kentaro Browse this author →KAKEN DB
Saasa, Ngonda Browse this author
Sakai, Misuki Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Keywords: Tick-borne encephalitis virus
Fc fusion protein
Issue Date: Apr-2014
Publisher: Elsevier
Journal Title: Diagnostic Microbiology and Infectious Disease
Volume: 78
Issue: 4
Start Page: 373
End Page: 378
Publisher DOI: 10.1016/j.diagmicrobio.2013.12.014
PMID: 24492026
Abstract: Current diagnostic tests for tick-borne encephalitis virus (TBEV) infections require high-biosafety facilities for antigen preparation and can cross-react with other flaviviruses. There is, therefore, a need to develop safe, inexpensive serodiagnostic tools with high specificity and sensitivity. In this study, a recombinant plasmid that expresses the membrane and envelope (E) proteins of TBEV fused to the Fc domain of rabbit IgG was constructed and expressed in mammalian cells. The E-Fc proteins were secreted as soluble homodimers, which retained reactivity with anti-TBEV and anti-rabbit IgG antibodies. The E-Fc proteins were then used to develop an enzyme-linked immunosorbent assay (ELISA) to detect TBEV antibodies in rodent and human sera. Compared with the neutralization test, the ELISA had over 90% sensitivity and specificity. In addition, the assay showed no cross-reactivity with Japanese encephalitis virus antibodies. These findings suggest that the E-Fc ELISA may be a useful tool for TBEV serodiagnosis. (C) 2014 Elsevier Inc. All rights reserved.
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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