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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins
| Title: | Development of a tick-borne encephalitis serodiagnostic ELISA using recombinant Fc-antigen fusion proteins |
| Authors: | Chidumayo, Nozyechi N. Browse this author | | Yoshii, Kentaro Browse this author →KAKEN DB | | Saasa, Ngonda Browse this author | | Sakai, Misuki Browse this author | | Kariwa, Hiroaki Browse this author →KAKEN DB |
| Keywords: | Tick-borne encephalitis virus | | Fc fusion protein | | Diagnosis | | ELISA |
| Issue Date: | Apr-2014 |
| Publisher: | Elsevier |
| Journal Title: | Diagnostic Microbiology and Infectious Disease |
| Volume: | 78 |
| Issue: | 4 |
| Start Page: | 373 |
| End Page: | 378 |
| Publisher DOI: | 10.1016/j.diagmicrobio.2013.12.014 |
| PMID: | 24492026 |
| Abstract: | Current diagnostic tests for tick-borne encephalitis virus (TBEV) infections require high-biosafety facilities for antigen preparation and can cross-react with other flaviviruses. There is, therefore, a need to develop safe, inexpensive serodiagnostic tools with high specificity and sensitivity. In this study, a recombinant plasmid that expresses the membrane and envelope (E) proteins of TBEV fused to the Fc domain of rabbit IgG was constructed and expressed in mammalian cells. The E-Fc proteins were secreted as soluble homodimers, which retained reactivity with anti-TBEV and anti-rabbit IgG antibodies. The E-Fc proteins were then used to develop an enzyme-linked immunosorbent assay (ELISA) to detect TBEV antibodies in rodent and human sera. Compared with the neutralization test, the ELISA had over 90% sensitivity and specificity. In addition, the assay showed no cross-reactivity with Japanese encephalitis virus antibodies. These findings suggest that the E-Fc ELISA may be a useful tool for TBEV serodiagnosis. (C) 2014 Elsevier Inc. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/56324 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 好井 健太朗
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