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The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.

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Title: The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.
Authors: Kang, Sung-Sik Browse this author
Ofuji, Sosuke Browse this author
Imai, Kei Browse this author →KAKEN DB
Huang, Weiping Browse this author
Koyama, Keisuke Browse this author
Yanagawa, Yojiro Browse this author
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: Bovine
Embryonic Development
Droplet
IVC
WOW
Issue Date: May-2014
Publisher: Cambridge University Press
Journal Title: Zygote
Volume: 23
Issue: 3
Start Page: 412
End Page: 415
Publisher DOI: 10.1017/S096719941400001X
PMID: 24598303
Abstract: The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.
Rights: © Cambridge University Press 2014
Type: article
URI: http://hdl.handle.net/2115/56333
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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