|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.
| Title: | The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development. |
| Authors: | Kang, Sung-Sik Browse this author | | Ofuji, Sosuke Browse this author | | Imai, Kei Browse this author →KAKEN DB | | Huang, Weiping Browse this author | | Koyama, Keisuke Browse this author | | Yanagawa, Yojiro Browse this author | | Takahashi, Yoshiyuki Browse this author →KAKEN DB | | Nagano, Masashi Browse this author →KAKEN DB |
| Keywords: | Bovine | | Embryonic Development | | Droplet | | IVC | | WOW |
| Issue Date: | May-2014 |
| Publisher: | Cambridge University Press |
| Journal Title: | Zygote |
| Volume: | 23 |
| Issue: | 3 |
| Start Page: | 412 |
| End Page: | 415 |
| Publisher DOI: | 10.1017/S096719941400001X |
| PMID: | 24598303 |
| Abstract: | The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish. |
| Rights: | © Cambridge University Press 2014 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/56333 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 永野 昌志
|
