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Aging-related changes in in vitro-matured bovine oocytes: oxidative stress, mitochondrial activity and ATP content after nuclear maturation.

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Title: Aging-related changes in in vitro-matured bovine oocytes: oxidative stress, mitochondrial activity and ATP content after nuclear maturation.
Authors: Koyama, Keisuke Browse this author
Kang, Sung-Sik Browse this author
Huang, Weiping Browse this author
Yanagawa, Yojiro Browse this author
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: Aging
ATP
Bovine oocytes
Mitochondria
ROS
Issue Date: Apr-2014
Publisher: 日本繁殖生物学会
Journal Title: The Journal of reproduction and development
Volume: 60
Issue: 2
Start Page: 136
End Page: 142
PMID: 24492658
Abstract: The objective of this research was to clarify the aging-related changes in in vitro-matured bovine oocytes. Firstly, we examined the fertilization and embryonic development of bovine oocytes after 22 and 30-34 h of in vitro maturation (IVM). The oocytes after 30-34 h of IVM (penetrated by sperm at around 40 h after starting IVM) showed a lower developmental rate to blastocysts (P<0.01), although normal fertilization rates were similar regardless of IVM duration. In the next experiment, reactive oxygen species (ROS), mitochondrial activity and ATP content in oocytes after 20, 30 and 40 h of IVM were examined. The lowest level of ROS was found in the group subjected to 30 h of IVM. The mitochondrial activity and ATP content in the group subjected to 40 h of IVM were higher than in the group subjected to 20 h of IVM (P<0.01), and those in the group subjected to 30 h of IVM showed intermediate values. Thereafter, the mitochondrial activities at 3 days after in vitro fertilization in embryos derived from the oocytes subjected to 22 and 34 h of IVM were evaluated. In the group subjected to 34 h of IVM, high-polarized mitochondria were frequently observed at the periphery of blastomeres. The present results suggest that high mitochondrial activity observed in oocytes after prolonged IVM culture and localization of high-polarized mitochondria at the periphery of blastomeres during early embryonic development may be associated with the low developmental competence in aged bovine oocytes.
Type: article
URI: http://hdl.handle.net/2115/56343
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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