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Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation

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Title: Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation
Authors: Tamura, Masato Browse this author →KAKEN DB
Uyama, Maki Browse this author
Sugiyama, Yuri Browse this author
Sato, Mari Browse this author →KAKEN DB
Keywords: miRNA
Issue Date: Dec-2013
Publisher: Spandidos Publications
Journal Title: Molecular Medicine Reports
Volume: 8
Issue: 6
Start Page: 1807
End Page: 1811
Publisher DOI: 10.3892/mmr.2013.1713
PMID: 24100761
Abstract: The canonical Wnt signaling pathway is crucial for the regulation of bone mass in humans and for the development of osteoblasts. MicroRNAs (miRs) represent a class of non-coding RNAs, similar to 22 nucleotides in length, that regulate gene expression by targeting mRNAs for cleavage or translational repression. Several previous studies have demonstrated the involvement of miRNAs in modulating gene expression in osteoblasts and regulating osteoblast differentiation. In the present study, microRNA profiling was conducted using Wnt3a-C2C12 cells; C2C12 cells were transfected with a Wnt3a expression plasmid to activate canonical Wnt signaling. miR-34b-5p and miR-34c were identified to be upregulated by the activation of canonical Wnt signaling in C2C12 cells. Expression of mature miR-34b/c increased from low levels at day 0 to maximum levels at day 28 of MC3T3-E1 cell differentiation. To analyze the effects of these miRNAs on osteoblast differentiation, an antisense inhibitor was transfected into MC3T3-E1 cells and osteoblast-related gene expression was investigated. Knockdown of miR34b/c enhanced osteocalcin mRNA expression; however, alkaline phosphatase mRNA expression and activity were decreased by miR34b/c inhibition. These results indicated that miR-34b/c regulates gene expression by targeting regulators of the osteogenic pathways and thereby contributes to osteoblast differentiation.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村 正人

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