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N-linked glycan in tick-borne encephalitis virus envelope protein affects viral secretion in mammalian cells, but not in tick cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/56524

Title: N-linked glycan in tick-borne encephalitis virus envelope protein affects viral secretion in mammalian cells, but not in tick cells
Authors: Yoshii, Kentaro Browse this author →KAKEN DB
Yanagihara, Natsumi Browse this author
Ishizuka, Mariko Browse this author
Sakai, Mizuki Browse this author
Kariwa, Hiroaki Browse this author →KAKEN DB
Issue Date: Oct-2013
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 94
Start Page: 2249
End Page: 2258
Publisher DOI: 10.1099/vir.0.055269-0
PMID: 23824303
Abstract: Tick-borne encephalitis virus (TBEV) is a zoonotic disease agent that causes severe encephalitis in humans. The envelope protein E of TBEV has one N-linked glycosylation consensus sequence, but little is known about the biological function of the N-linked glycan. In this study, the function of protein E glycosylation was investigated using recombinant TBEV with or without the protein E N-linked glycan. Virion infectivity was not affected after removing the N-linked glycans using N-glycosidase F. In mammalian cells, loss of glycosylation affected the conformation of protein E during secretion, reducing the infectivity of secreted virions. Mice subcutaneously infected with TBEV lacking protein E glycosylation showed no signs of disease, and viral multiplication in peripheral organs was reduced relative to that with the parental virus. In contrast, loss of glycosylation did not affect the secretory process of infectious virions in tick cells. Furthermore, inhibition of transport to the Golgi apparatus affected TBEV secretion in mammalian cells, but not in tick cells, indicating that TBEV was secreted through an unidentified pathway after synthesis in endoplasmic reticulum in tick cells. These results increase our understanding of the molecular mechanisms of TBEV maturation.
Type: article (author version)
URI: http://hdl.handle.net/2115/56524
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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