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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
N-linked glycan in tick-borne encephalitis virus envelope protein affects viral secretion in mammalian cells, but not in tick cells
| Title: | N-linked glycan in tick-borne encephalitis virus envelope protein affects viral secretion in mammalian cells, but not in tick cells |
| Authors: | Yoshii, Kentaro Browse this author →KAKEN DB | | Yanagihara, Natsumi Browse this author | | Ishizuka, Mariko Browse this author | | Sakai, Mizuki Browse this author | | Kariwa, Hiroaki Browse this author →KAKEN DB |
| Issue Date: | Oct-2013 |
| Publisher: | Society for General Microbiology |
| Journal Title: | Journal of General Virology |
| Volume: | 94 |
| Start Page: | 2249 |
| End Page: | 2258 |
| Publisher DOI: | 10.1099/vir.0.055269-0 |
| PMID: | 23824303 |
| Abstract: | Tick-borne encephalitis virus (TBEV) is a zoonotic disease agent that causes severe encephalitis in humans. The envelope protein E of TBEV has one N-linked glycosylation consensus sequence, but little is known about the biological function of the N-linked glycan. In this study, the function of protein E glycosylation was investigated using recombinant TBEV with or without the protein E N-linked glycan. Virion infectivity was not affected after removing the N-linked glycans using N-glycosidase F. In mammalian cells, loss of glycosylation affected the conformation of protein E during secretion, reducing the infectivity of secreted virions. Mice subcutaneously infected with TBEV lacking protein E glycosylation showed no signs of disease, and viral multiplication in peripheral organs was reduced relative to that with the parental virus. In contrast, loss of glycosylation did not affect the secretory process of infectious virions in tick cells. Furthermore, inhibition of transport to the Golgi apparatus affected TBEV secretion in mammalian cells, but not in tick cells, indicating that TBEV was secreted through an unidentified pathway after synthesis in endoplasmic reticulum in tick cells. These results increase our understanding of the molecular mechanisms of TBEV maturation. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/56524 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 好井 健太朗
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