|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Molecular characterization of Mycobacterium tuberculosis isolates from elephants of Nepal
| Title: | Molecular characterization of Mycobacterium tuberculosis isolates from elephants of Nepal |
| Authors: | Paudel, Sarad Browse this author | | Mikota, Susan K. Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Gairhe, Kamal P. Browse this author | | Maharjan, Bhagwan Browse this author | | Thapa, Jeewan Browse this author | | Poudel, Ajay Browse this author | | Shimozuru, Michito Browse this author | | Suzuki, Yasuhiko Browse this author →KAKEN DB | | Tsubota, Toshio Browse this author →KAKEN DB |
| Keywords: | Asian elephants | | Mycobacterium tuberculosis | | Multi-locus variable number tandem repeat analysis (MLVA) | | Spoligotyping |
| Issue Date: | May-2014 |
| Publisher: | Elsevier |
| Journal Title: | Tuberculosis |
| Volume: | 94 |
| Issue: | 3 |
| Start Page: | 287 |
| End Page: | 292 |
| Publisher DOI: | 10.1016/j.tube.2013.12.008 |
| PMID: | 24566285 |
| Abstract: | Mycobacterium tuberculosis was cultured from the lung tissues of 3 captive elephants in Nepal that died with extensive lung lesions. Spoligotyping, TbD1 detection and multi-locus variable number of tandem repeat analysis (MLVA) results suggested 3 isolates belonged to a specific lineage of Indo-Oceanic clade, EAI5 SIT 138. One of the elephant isolates had a new synonymous single nucleotide polymorphism (SNP) T231C in the gyrA sequence, and the same SNP was also found in human isolates in Nepal. MLVA results and transfer history of the elephants suggested that 2 of them might be infected with M. tuberculosis from the same source. These findings indicated the source of M. tuberculosis infection of those elephants were local residents, presumably their handlers. Further investigation including detailed genotyping of elephant and human isolates is needed to clarify the infection route and eventually prevent the transmission of tuberculosis to susceptible hosts. (C) 2014 Elsevier Ltd. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/56576 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 坪田 敏男
|
