|Title: ||Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide|
|Authors: ||Miyaji, Hirofumi Browse this author →KAKEN DB|
|Kanayama, Izumi Browse this author|
|Takita, Hiroko Browse this author →KAKEN DB|
|Nishida, Erika Browse this author|
|Tsuji, Maiko Browse this author|
|Fugetsu, Bunshi Browse this author →KAKEN DB|
|Sun, Ling Browse this author|
|Inoue, Kana Browse this author|
|Ibara, Asako Browse this author|
|Akasaka, Tsukasa Browse this author →KAKEN DB|
|Sugaya, Tsutomu Browse this author →KAKEN DB|
|Kawanami, Masamitsu Browse this author →KAKEN DB|
|Issue Date: ||11-Jul-2014|
|Publisher: ||Dove Medical Press|
|Journal Title: ||International Journal of Nanomedicine|
|Start Page: ||3363|
|End Page: ||3373|
|Publisher DOI: ||10.2147/IJN.S62342|
|Abstract: ||Graphene oxide (GO) is a single layer carbon sheet with a thickness of less than 1 nm. GO has good dispersibility due to surface modifications with numerous functional groups. Reduced graphene oxide (RGO) is produced via the reduction of GO, and has lower dispersibility. We examined the bioactivity of GO and RGO films, and collagen scaffolds coated with GO and RGO.
METHODS:GO and RGO films were fabricated on a culture dish. Some GO films were chemically reduced using either ascorbic acid or sodium hydrosulfite solution, resulting in preparation of RGO films. The biological properties of each film were evaluated by scanning electron microscopy (SEM), atomic force microscopy, calcium adsorption tests, and MC3T3-E1 cell seeding. Subsequently, GO- and RGO-coated collagen scaffolds were prepared and characterized by SEM and compression tests. Each scaffold was implanted into subcutaneous tissue on the backs of rats. Measurements of DNA content and cell ingrowth areas of implanted scaffolds were performed 10 days post-surgery.
RESULTS: The results show that GO and RGO possess different biological properties. Calcium adsorption and alkaline phosphatase activity were strongly enhanced by RGO, suggesting that RGO is effective for osteogenic differentiation. SEM showed that RGO-modified collagen scaffolds have rough, irregular surfaces. The compressive strengths of GO- and RGO-coated scaffolds were approximately 1.7-fold and 2.7-fold greater, respectively, when compared with the non-coated scaffold. Tissue ingrowth rate was 39% in RGO-coated scaffolds, as compared to 20% in the GO-coated scaffold and 16% in the non-coated scaffold.
CONCLUSION: In summary, these results suggest that GO and RGO coatings provide different biological properties to collagen scaffolds, and that RGO-coated scaffolds are more bioactive than GO-coated scaffolds.|
|Appears in Collections:||歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)|