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Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide

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Title: Comparative study of bioactivity of collagen scaffolds coated with graphene oxide and reduced graphene oxide
Authors: Miyaji, Hirofumi Browse this author →KAKEN DB
Kanayama, Izumi Browse this author
Takita, Hiroko Browse this author →KAKEN DB
Nishida, Erika Browse this author
Tsuji, Maiko Browse this author
Fugetsu, Bunshi Browse this author →KAKEN DB
Sun, Ling Browse this author
Inoue, Kana Browse this author
Ibara, Asako Browse this author
Akasaka, Tsukasa Browse this author →KAKEN DB
Sugaya, Tsutomu Browse this author →KAKEN DB
Kawanami, Masamitsu Browse this author →KAKEN DB
Keywords: GO
cell ingrowth
regenerative scaffold
tissue engineering
Issue Date: 11-Jul-2014
Publisher: Dove Medical Press
Journal Title: International Journal of Nanomedicine
Volume: 9
Start Page: 3363
End Page: 3373
Publisher DOI: 10.2147/IJN.S62342
PMID: 25050063
Abstract: Graphene oxide (GO) is a single layer carbon sheet with a thickness of less than 1 nm. GO has good dispersibility due to surface modifications with numerous functional groups. Reduced graphene oxide (RGO) is produced via the reduction of GO, and has lower dispersibility. We examined the bioactivity of GO and RGO films, and collagen scaffolds coated with GO and RGO. METHODS:GO and RGO films were fabricated on a culture dish. Some GO films were chemically reduced using either ascorbic acid or sodium hydrosulfite solution, resulting in preparation of RGO films. The biological properties of each film were evaluated by scanning electron microscopy (SEM), atomic force microscopy, calcium adsorption tests, and MC3T3-E1 cell seeding. Subsequently, GO- and RGO-coated collagen scaffolds were prepared and characterized by SEM and compression tests. Each scaffold was implanted into subcutaneous tissue on the backs of rats. Measurements of DNA content and cell ingrowth areas of implanted scaffolds were performed 10 days post-surgery. RESULTS: The results show that GO and RGO possess different biological properties. Calcium adsorption and alkaline phosphatase activity were strongly enhanced by RGO, suggesting that RGO is effective for osteogenic differentiation. SEM showed that RGO-modified collagen scaffolds have rough, irregular surfaces. The compressive strengths of GO- and RGO-coated scaffolds were approximately 1.7-fold and 2.7-fold greater, respectively, when compared with the non-coated scaffold. Tissue ingrowth rate was 39% in RGO-coated scaffolds, as compared to 20% in the GO-coated scaffold and 16% in the non-coated scaffold. CONCLUSION: In summary, these results suggest that GO and RGO coatings provide different biological properties to collagen scaffolds, and that RGO-coated scaffolds are more bioactive than GO-coated scaffolds.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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