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pp32r1 controls the decay of the RNA- binding protein HuR

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Title: pp32r1 controls the decay of the RNA- binding protein HuR
Authors: Imamachi, Kenji Browse this author
Higashino, Fumihiro Browse this author →KAKEN DB
Kitamura, Tetsuya Browse this author →KAKEN DB
Kakuguchi, Wataru Browse this author
Yanagawa-Matsuda, Aya Browse this author →KAKEN DB
Ishikawa, Makoto Browse this author →KAKEN DB
Kitagawa, Yoshimasa Browse this author →KAKEN DB
Totsuka, Yasunori Browse this author →KAKEN DB
Shindoh, Masanobu Browse this author →KAKEN DB
Keywords: pp32r1
AU-rich element
Issue Date: Mar-2014
Publisher: Spandidos Publications
Journal Title: Oncology Reports
Volume: 31
Issue: 3
Start Page: 1103
End Page: 1108
Publisher DOI: 10.3892/or.2013.2956
PMID: 24398589
Abstract: pp32 is a tumor suppressor and is one of the associated proteins of the RNA-binding protein HuR. The pp32-HuR complex is exported to the cytoplasm of cells under stress conditions, and HuR is degraded by caspases in the cytoplasm. In the present study, we examined the role of pp32r1, a member of the pp32 family that has oncogenic properties, in the decay of HuR. pp32r1 was found to be abundantly expressed in cancer cells, and overexpression of pp32r1 induced colony formation in soft-agar. pp32r1 was expressed in both the nucleus and cytoplasm, whereas pp32 was predominantly localized in the nucleus. Even with lethal stress such as staurosporine (STS), HuR in the cytoplasm was never downregulated, and caspase-3 activity was inhibited when cells expressed pp32r1. pp32r1 bound to HuR without interacting with pp32. In cancer cells, HuR survived in the cytoplasm of cells overexpressing pp32r1, although HuR was not expressed in the cytoplasm of pp32-expressing cells, similar to lethal stress conditions. Taken together, these results indicate that pp32r1 binds to HuR to avoid the caspase-mediated decay of HuR in the cytoplasm of cells. We suggest that this function contributes to the oncogenic activity of pp32r1.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 東野 史裕

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