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Volume 62 Number 3 >

Organ-specific changes in norepinephrine turnover against various stress conditions in thermoneutral mice

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.62.3.117

Title: Organ-specific changes in norepinephrine turnover against various stress conditions in thermoneutral mice
Authors: Teramura, Yasufumi Browse this author
Terao, Akira Browse this author →KAKEN DB
Okada, Yuko Browse this author
Tomida, Junichi Browse this author
Okamatsu-Ogura, Yuko Browse this author →KAKEN DB
Kimura, Kazuhiro Browse this author →KAKEN DB
Keywords: sympathetic nervous system
adrenocortical system
cold exposure
immobilization
lipopolysaccharide
Issue Date: Aug-2014
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 62
Issue: 3
Start Page: 117
End Page: 127
Abstract: The effects of three stressors of different categories, namely cold exposure, immobilization, and lipopolysaccharide (LPS) treatment, on sympathetic nerve activity were examined by assessing its biochemical index norepinephrine (NE) turnover in peripheral organs of C57BL/6 mice. NE turnover was assessed by measuring the decrease in the organ NE concentration 3 h after inhibition of catecholamine biosynthesis with α-methyl-p-tyrosine. NE turnover in brown adipose tissue (BAT) in the room temperature (23°C) control group was as high as that in the cold exposure (4°C) group. Similarly, the mRNA level of the thermogenic marker uncoupling protein 1 (UCP1) in the room temperature control group was as high as that in the cold exposure group. As sympathetic stimulation upregulates the UCP1 mRNA level, we thought that sympathetic nerve tonus in BAT was already accelerated at room temperature. To exclude factors affecting basal sympathetic nerve activity, mice housed at thermoneutral temperature (30°C) were used as controls for the subsequent experiments. In this condition, cold exposure accelerated NE turnover in the BAT, as well as heart and pancreas. The corticosterone level showed a higher trend in the cold exposure group in comparison to the control group. Immobilization accelerated NE turnover in the spleen, pancreas, and white adipose tissue and elevated the corticosterone level. LPS (3 mg/kg, i.p.) did not affect NE turnover in all peripheral organs but elevated the corticosterone level. In summary, the sympathetic nervous and adrenocortical responses to three stressors differed greatly. In particular, sympathetic responses showed clear organ-specific acceleration patterns. This important feature may improve our understanding of the multiplicity of biological responses.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/56895
Appears in Collections:Japanese Journal of Veterinary Research > Volume 62 Number 3

Submitter: 獣医学部図書室

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