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Volume 62 Number 3 >

Effects of in vitro-growth culture duration on fertilizability of bovine growing oocytes and proliferation of cells surrounding oocytes

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.62.3.135

Title: Effects of in vitro-growth culture duration on fertilizability of bovine growing oocytes and proliferation of cells surrounding oocytes
Authors: Huang, Weiping Browse this author
Kang, Sung-Sik Browse this author
Yanagawa, Yojiro Browse this author
Yang, Yinghua Browse this author
Takahashi, Yoshiyuki Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: early antral follicles
fertilization
IVG duration
Issue Date: Aug-2014
Publisher: Graduate School of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 62
Issue: 3
Start Page: 135
End Page: 141
Abstract: The effects of in vitro-growth (IVG) culture duration (12 and 14 days) on the fertilizability and the developmental ability of IVG oocytes matured with pre-maturational culture, and on the proliferation of cells surrounding oocytes during IVG culture were examined. The fertilization and cleavage rates of 12- and 14-day IVG oocytes were similar; however, 14-day IVG oocytes showed lower blastocyst development rate than 12-day IVG oocytes. In addition, the number of cells surrounding oocytes increased until 12 days of IVG, but decreased at 14 days. The results indicated that the extension of IVG period beyond 12 days impaired the proliferation of cells surrounding oocytes, leading to the decrease of developmental ability but not fertilizability of 14-day IVG oocytes.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/56897
Appears in Collections:Japanese Journal of Veterinary Research > Volume 62 Number 3

Submitter: 獣医学部図書室

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