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Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer

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Title: Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer
Authors: Kojima, Hisaya Browse this author →KAKEN DB
Tokizawa, Riho Browse this author
Kogure, Kouhei Browse this author
Kobayashi, Yuki Browse this author
Itoh, Masayuki Browse this author →KAKEN DB
Shiah, Fuh-Kwo Browse this author
Okuda, Noboru Browse this author →KAKEN DB
Fukui, Manabu Browse this author →KAKEN DB
Issue Date: 25-Jul-2014
Publisher: Nature Publishing Group
Journal Title: Scientific Reports
Volume: 4
Start Page: 5728
Publisher DOI: 10.1038/srep05728
Abstract: Methane-oxidizing bacteria (MOB) gain energy from the oxidation of methane and may play important roles in freshwater ecosystems. In this study, the community structure of planktonic MOB was investigated in a subtropical reservoir. Bacterial community structure was investigated through the analysis of the 16S rRNA gene. Three groups of phylogenetically distinct MOB were detected in the clone libraries of polymerase chain reaction products obtained with universal primers. The groups belonged to the class Gammaproteobacteria, the class Alphaproteobacteria, and the candidate phylum NC10. The last group, which consists of close relatives of the nitrite reducer 'Candidatus Methylomirabilis oxyfera', was frequently detected in the clone libraries of deep-water environments. The presence of 3 groups of MOB in deep water was also shown by a cloning analysis of the pmoA gene encoding particulate methane monooxygenase. The dominance of 'M. oxyfera'-like organisms in deep water was confirmed by catalyzed reporter depositionfluorescence in situ hybridization, in which cells stained with a specific probe accounted for 16% of total microbial cells. This is the first study to demonstrate that close relatives of the nitrite reducer can be major component of planktonic MOB community which may affect carbon flow in aquatic ecosystems.
Rights: http://creativecommons.org/licenses/by-nc-sa/4.0/
Type: article
URI: http://hdl.handle.net/2115/57004
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小島 久弥

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