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Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel

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Title: Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel
Authors: Ishida, Sumire Browse this author
Tanaka, Ryosuke Browse this author
Yamaguchi, Naoya Browse this author
Ogata, Genki Browse this author
Mizutani, Takeomi Browse this author →KAKEN DB
Kawabata, Kazushige Browse this author
Haga, Hisashi Browse this author →KAKEN DB
Issue Date: 29-Aug-2014
Publisher: Public Library of Science
Journal Title: PLOS ONE
Volume: 9
Issue: 8
Start Page: e99655
Publisher DOI: 10.1371/journal.pone.0099655
Abstract: Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-beta 1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-beta 1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/57093
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 芳賀 永

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