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Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel
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Title: | Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel |
Authors: | Ishida, Sumire Browse this author | Tanaka, Ryosuke Browse this author | Yamaguchi, Naoya Browse this author | Ogata, Genki Browse this author | Mizutani, Takeomi Browse this author →KAKEN DB | Kawabata, Kazushige Browse this author | Haga, Hisashi Browse this author →KAKEN DB |
Issue Date: | 29-Aug-2014 |
Publisher: | Public Library of Science |
Journal Title: | PLOS ONE |
Volume: | 9 |
Issue: | 8 |
Start Page: | e99655 |
Publisher DOI: | 10.1371/journal.pone.0099655 |
Abstract: | Lumen formation is important for morphogenesis; however, an unanswered question is whether it involves the collective migration of epithelial cells. Here, using a collagen gel overlay culture method, we show that Madin-Darby canine kidney cells migrated collectively and formed a luminal structure in a collagen gel. Immediately after the collagen gel overlay, an epithelial sheet folded from the periphery, migrated inwardly, and formed a luminal structure. The inhibition of integrin-beta 1 or Rac1 activity decreased the migration rate of the peripheral cells after the sheets folded. Moreover, lumen formation was perturbed by disruption of apical-basolateral polarity induced by transforming growth factor-beta 1. These results indicate that cell migration and cell polarity play an important role in folding. To further explore epithelial sheet folding, we developed a computer-simulated mechanical model based on the rigidity of the extracellular matrix. It indicated a soft substrate is required for the folding movement. |
Rights: | http://creativecommons.org/licenses/by/4.0/ |
Type: | article |
URI: | http://hdl.handle.net/2115/57093 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 芳賀 永
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