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Genetic characterization of the progeny of a pair of the tetraploid silver crucian carp Carassius auratus langsdorfii

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Title: Genetic characterization of the progeny of a pair of the tetraploid silver crucian carp Carassius auratus langsdorfii
Other Titles: 四倍体ギンブナ雌雄ペア子孫の遺伝学的特徴
Authors: Dong, Jie1 Browse this author
Murakami, Masaru2 Browse this author →KAKEN DB
Fujimoto, Takafumi3 Browse this author
Yamaha, Etsuro4 Browse this author →KAKEN DB
Arai, Katsutoshi5 Browse this author →KAKEN DB
Authors(alt): 董, 捷1
村上, 賢2
藤本, 貴史3
山羽, 悦郎4
荒井, 克俊5
Keywords: Silver crucian carp
Flow cytometry
RAPD markers
Issue Date: Nov-2013
Publisher: The Japanese Society of Fisheries Science
Journal Title: Fisheries Science
Volume: 79
Issue: 6
Start Page: 935
End Page: 941
Publisher DOI: 10.1007/s12562-013-0674-x
Abstract: Silver crucian carp Carassius auratus langsdorfii comprises a diploid-polyploid complex in wild Japanese populations. Bisexually reproducing diploids are sympatrically distributed with gynogenetically developing triploids and tetraploids. Triploid and tetraploid males are very rare among Japanese silver crucian carp due to their gynogenetic reproduction. We examined the genetic characteristics of progeny that arose in a tank by natural spawning of a tetraploid silver crucian carp pair. The ploidy status of 120 samples randomly collected from these progeny was determined to be tetraploid by DNA content flow cytometry. DNA fingerprints from a random amplified polymorphic DNA assay indicated that almost all the progeny examined had genotypes identical to the maternal tetraploid female with no paternally derived fragments. Selected specimens’ cytogenetic analyses revealed that the progeny examined had tetraploid chromosome numbers, categorized into 40 metacentric, 80 submetacentric, and 80 subtelocentric or telocentric chromosomes, which were arranged into quartets and six supernumerary microchromosomes. Fluorescence in situ hybridization signals were detected in four homologous chromosomes in all analyzed metaphases prepared from diploid goldfish specimens. Contrary, tetraploid silver crucian carp gave eight rDNA signals. These results suggest that gynogenetic development in eggs spawned by tetraploid females should be triggered by tetraploid males’ homospecific sperm.
四倍体ギンブナ雌雄の自然産卵により得た子孫のDNA 量を調べたところ、全個体が四倍体であった。さらにRAPD-PCR 法により分析したところ、子孫と母親の間で電気泳動像は一例を除き同一で、かつ父親の遺伝的影響は認められなかった。子孫は染色体数4n=206、核型40M+80SM+80ST/T+6m(微小染色体)を示し、5.8S+28SrDNA をプローブとしたFISH では、二倍体キンギョの4 シグナルに対して8 シグナルを示した。以上の結果は、四倍体の精子によっても、四倍体の卵の雌性発生が生じ、子孫は四倍体クローンとなったことを示す。
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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