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A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/57285

Title: A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis
Authors: Uji, Toshiki Browse this author →KAKEN DB
Hirata, Ryo Browse this author
Fukuda, Satoru Browse this author
Mizuta, Hiroyuki Browse this author →KAKEN DB
Saga, Naotsune Browse this author →KAKEN DB
Keywords: Pyropia yezoensis
Red alga
Selection marker
Stable transformation
Issue Date: Jun-2014
Publisher: Springer
Journal Title: Marine Biotechnology
Volume: 16
Issue: 3
Start Page: 251
End Page: 255
Publisher DOI: 10.1007/s10126-013-9549-5
Abstract: Marine macroalgae play an important role in marine coastal ecosystems and are widely used as sea vegetation foodstuffs and for industrial purposes. Therefore, there have been increased demands for useful species and varieties of these macroalgae. However, genetic transformation in macroalgae has not yet been established. We have developed a dominant selection marker for stable nuclear transformation in the red macroalga Pyropia yezoensis. We engineered the coding region of the aminoglycoside phosphotransferase gene aph7aEuro(3) from Streptomyces hygroscopicus to adapt codon usage of the nuclear genes of P. yezoensis. We designated this codon-optimized aph7aEuro(3) gene as PyAph7. After bombarding P. yezoensis cells with plasmids containing PyAph7 under the control of their endogenous promoter, 1.9 thalli (or individuals) of hygromycin-resistant strains were isolated from a 10-mm square piece of the bombarded thallus. These transformants were stably maintained throughout the asexual life cycle. Stable expression of PyAph7was verified using Southern blot analysis and genomic PCR and RT-PCR analyses. PyAph7 proved to be a new versatile tool for stable nuclear transformation in P. yezoensis.
Rights: The final publication is available at link.springer.com
Type: article
URI: http://hdl.handle.net/2115/57285
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宇治 利樹

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