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Anchoring protein crystals to mounting loops with hydrogel using inkjet technology

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/57793

Title: Anchoring protein crystals to mounting loops with hydrogel using inkjet technology
Authors: Shinoda, Akira Browse this author
Tanaka, Yoshikazu Browse this author →KAKEN DB
Yao, Min Browse this author →KAKEN DB
Tanaka, Isao Browse this author →KAKEN DB
Issue Date: Nov-2014
Publisher: Wiley-Blackwell
Journal Title: Acta crystallographica section D : biological crystallography
Volume: 70
Issue: 11
Start Page: 2794
End Page: 2799
Publisher DOI: 10.1107/S139900471401476X
PMID: 25372671
Abstract: X-ray crystallography is an important technique for structure-based drug discovery, mainly because it is the only technique that can reveal whether a ligand binds to the target protein as well as where and how it binds. However, ligand screening by X-ray crystallography involves a crystal-soaking experiment, which is usually performed manually. Thus, the throughput is not satisfactory for screening large numbers of candidate ligands. In this study, a technique to anchor protein crystals to mounting loops by using gel and inkjet technology has been developed; the method allows soaking of the mounted crystals in ligand-containing solution. This new technique may assist in the design of a fully automated drug-screening pipeline.
Type: article
URI: http://hdl.handle.net/2115/57793
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 勲

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