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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Anchoring protein crystals to mounting loops with hydrogel using inkjet technology
| Title: | Anchoring protein crystals to mounting loops with hydrogel using inkjet technology |
| Authors: | Shinoda, Akira Browse this author | | Tanaka, Yoshikazu Browse this author →KAKEN DB | | Yao, Min Browse this author →KAKEN DB | | Tanaka, Isao Browse this author →KAKEN DB |
| Issue Date: | Nov-2014 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Acta crystallographica section D : biological crystallography |
| Volume: | 70 |
| Issue: | 11 |
| Start Page: | 2794 |
| End Page: | 2799 |
| Publisher DOI: | 10.1107/S139900471401476X |
| PMID: | 25372671 |
| Abstract: | X-ray crystallography is an important technique for structure-based drug discovery, mainly because it is the only technique that can reveal whether a ligand binds to the target protein as well as where and how it binds. However, ligand screening by X-ray crystallography involves a crystal-soaking experiment, which is usually performed manually. Thus, the throughput is not satisfactory for screening large numbers of candidate ligands. In this study, a technique to anchor protein crystals to mounting loops by using gel and inkjet technology has been developed; the method allows soaking of the mounted crystals in ligand-containing solution. This new technique may assist in the design of a fully automated drug-screening pipeline. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/57793 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田中 勲
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