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Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA

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Title: Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA
Authors: Yamaguchi, Naoya Browse this author
Seshimo, Yuko Browse this author
Yoshimoto, Eri Browse this author
Ahn, Hong Il Browse this author
Ryu, Ki Hyun Browse this author
Choi, Jang Kyung Browse this author
Masuta, Chikara7 Browse this author →KAKEN DB
Authors(alt): 増田, 税7
Issue Date: 2005
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 86
Start Page: 2359
End Page: 2369
Publisher DOI: 10.1099/vir.0.81059-0
PMID: 16033984
Abstract: Five isolates of Cucumber mosaic virus (CMV) from Lilium sp. (lily), which were isolated from specimens in Japan, Korea and Taiwan, were unable to support satellite RNA (satRNA) accumulation. In order to map the CMV sequences that are involved in satRNA support, HL-CMV (Japanese lily isolate), Y-CMV (ordinary strain) and Y-satellite RNA (Y-sat) were used as the source material. The pseudorecombinants between Y-CMV and HL-CMV revealed that RNA1 was essential for satRNA replication in lily. The results of chimeric constructs and various mutations showed that two amino acid residues (at positions 876 and 891) in the 1a protein were the determinants for the inability of HL-CMV to support a satRNA. Specifically, Thr at position 876 had a more pronounced effect than Met at position 891. Specific changes in RNA sequence were also detected in the 3' terminus of Y-sat and these particular alterations allowed it to be supported by HL-CMV. It is believed that, through evolution, the adaptation of CMV to lily resulted in the introduction of amino acid changes in the 1a protein, changes that coincidentally affected the ability of lily CMV to support satRNAs.
Type: article
URI: http://hdl.handle.net/2115/5780
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 増田 税

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