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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway
| Title: | Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway |
| Authors: | Nagane, Masaki Browse this author | | Yasui, Hironobu Browse this author →KAKEN DB | | Sakai, Yuri Browse this author | | Yamamori, Tohru Browse this author →KAKEN DB | | Niwa, Koichi Browse this author →KAKEN DB | | Hattori, Yuichi Browse this author →KAKEN DB | | Kondo, Takashi Browse this author | | Inanami, Osamu Browse this author →KAKEN DB |
| Keywords: | Ionizing radiation | | endothelial nitric oxide synthase | | ATM | | HSP90 | | DNA damage response |
| Issue Date: | Feb-2015 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 456 |
| Issue: | 1 |
| Start Page: | 541 |
| End Page: | 546 |
| Publisher DOI: | 10.1016/j.bbrc.2014.12.002 |
| PMID: | 25498542 |
| Abstract: | In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/57811 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 稲波 修
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