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Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/57811

Title: Activation of eNOS in endothelial cells exposed to ionizing radiation involves components of the DNA damage response pathway
Authors: Nagane, Masaki Browse this author
Yasui, Hironobu Browse this author →KAKEN DB
Sakai, Yuri Browse this author
Yamamori, Tohru Browse this author →KAKEN DB
Niwa, Koichi Browse this author →KAKEN DB
Hattori, Yuichi Browse this author →KAKEN DB
Kondo, Takashi Browse this author
Inanami, Osamu Browse this author →KAKEN DB
Keywords: Ionizing radiation
endothelial nitric oxide synthase
ATM
HSP90
DNA damage response
Issue Date: Feb-2015
Publisher: Elsevier
Journal Title: Biochemical and Biophysical Research Communications
Volume: 456
Issue: 1
Start Page: 541
End Page: 546
Publisher DOI: 10.1016/j.bbrc.2014.12.002
PMID: 25498542
Abstract: In this study, the involvement of ataxia telangiectasia mutated (ATM) kinase and heat shock protein 90 (HSP90) in endothelial nitric oxide synthase (eNOS) activation was investigated in X-irradiated bovine aortic endothelial cells. The activity of nitric oxide synthase (NOS) and the phosphorylation of serine 1179 of eNOS (eNOS-Ser1179) were significantly increased in irradiated cells. The radiation-induced increases in NOS activity and eNOS-Ser1179 phosphorylation levels were significantly reduced by treatment with either an ATM inhibitor (Ku-60019) or an HSP90 inhibitor (geldanamycin). Geldanamycin was furthermore found to suppress the radiation-induced phosphorylation of ATM-Ser1181. Our results indicate that the radiation-induced eNOS activation in bovine aortic endothelial cells is regulated by ATM and HSP90.
Type: article (author version)
URI: http://hdl.handle.net/2115/57811
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 稲波 修

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