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Population genetic structure of Microdochium majus and Microdochium nivale associated with Fusarium head blight of wheat in Hokkaido, Japan

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Title: Population genetic structure of Microdochium majus and Microdochium nivale associated with Fusarium head blight of wheat in Hokkaido, Japan
Authors: Hayashi, Y. Browse this author
Kozawa, T. Browse this author
Aiuchi, D. Browse this author
Koike, M. Browse this author
Akino, S. Browse this author
Kondo, N. Browse this author →KAKEN DB
Keywords: Microdochium majus
Microdochium nivale
Genetic diversity
Population structure
ISSR marker
Fusarium head blight
Issue Date: Dec-2014
Publisher: Springer
Journal Title: European journal of plant pathology
Volume: 140
Issue: 4
Start Page: 787
End Page: 795
Publisher DOI: 10.1007/s10658-014-0509-3
Abstract: Microdochium majus and Microdochium nivale are two of fungal pathogens that cause Fusarium head blight (FHB) in wheat, and have also caused pink snow mold in eastern Hokkaido, Japan. With the aim of assessing levels of genetic variation and population structure, 172 isolates of these Microdochium species obtained from five populations of infected wheat seeds were first classified into each species using polymerase chain reaction (PCR) amplification with specific primers. In total 165 (95.9 % of all isolates) and seven isolates (six of Tokachi populations and one of Abashiri populations) were identified as M. majus and M. nivale, respectively, indicating that M. majus was predominant and the main causal pathogen of FHB in this area. Inter-simple sequence repeat (ISSR) analysis showed that the total genetic diversity was 0.023 when estimated by Nei's gene diversity index within the five populations dominated by M. majus. An AMOVA analysis also showed that 86.74 % of the total genetic variation was within populations and 13.26 % among populations. These results indicated that little genetic differentiation occurred among the five populations of M. majus. Based on the unweighted pair group method of cluster analysis using the ISSR data, all isolates were identified as one of eight haplotypes in M. majus or six haplotypes in M. nivale, allowing the construction of a dendrogram with two clades corresponding to each species. There was no correlation between the clustering of isolates and their geographic distribution on the tree. These findings show that migration is likely playing an important role in the population biology of M. majus, providing some support for the prediction of epidemics of fungicide resistant strains within populations of the FHB pathogen.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 近藤 則夫

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