| Title: | C-ERC/mesothelin provokes lymphatic invasion of colorectal adenocarcinoma |
| Authors: | Kawamata, Futoshi Browse this author |
| Homma, Shigenori Browse this author |
| Kamachi, Hirofumi Browse this author →KAKEN DB |
| Einama, Takahiro Browse this author |
| Kato, Yasutaka Browse this author |
| Tsuda, Masumi Browse this author →KAKEN DB |
| Tanaka, Shinya Browse this author →KAKEN DB |
| Maeda, Masahiro Browse this author |
| Kajino, Kazunori Browse this author |
| Hino, Okio Browse this author |
| Takahashi, Norihiko Browse this author |
| Kamiyama, Toshiya Browse this author →KAKEN DB |
| Nishihara, Hiroshi Browse this author →KAKEN DB |
| Taketomi, Akinobu Browse this author →KAKEN DB |
| Todo, Satoru Browse this author →KAKEN DB |
| Keywords: | C-ERC/mesothelin |
| Colorectal cancer |
| Lymphatic invasion |
| Issue Date: | Jan-2014 |
| Publisher: | Springer Japan |
| Journal Title: | Journal of gastroenterology |
| Volume: | 49 |
| Issue: | 1 |
| Start Page: | 81 |
| End Page: | 92 |
| Publisher DOI: | 10.1007/s00535-013-0773-6 |
| PMID: | 23512344 |
| Abstract: | Background Lymph node metastasis is a key event of colorectal cancer (CRC) progression. Mesothelin is expressed in various types of malignant tumor and associated with an unfavorable prognosis. The full-length mesothelin (Full-ERC) is cleaved by protease into membrane-bound C-ERC/mesothelin and N-ERC/mesothelin which is secreted into the blood. The aim of this study was to examine the biological role of mesothelin in CRC by clinicopathological analysis and in vitro lymphatic invasion assay. Methods Ninety-one cases of CRC specimens were immunohistochemically examined and the localization of mesothelin in luminal membrane and/or cytoplasm was also evaluated. Lymphatic invasion assay was also performed using the human CRC cell line, WiDr, which was transfected with Full-, N- and C-ERC/mesothelin expression plasmids (Full-WiDr, N-WiDr and C-WiDr). Results Immunohistochemically, "luminal membrane positive" of mesothelin was identified in 37.4 %, and correlated with lymphatic permeation and lymph node metastasis, but not with patients' prognosis. Interestingly, among the patients with lymph node metastasis (N = 38), "luminal membrane positive" of mesothelin significantly correlated with unfavorable patients' outcome. In addition, lymphatic invasion assay revealed that Full-WiDr and C-WiDr more significantly invaded human lymphatic endothelial cells than the Mock-WiDr (P < 0.01). Conclusion The luminal membrane expression of mesothelin was associated with unfavorable prognosis of CRC patients with lymph nodemetastasis. Moreover, this is the first report to prove the biological function of C-ERC/mesothelin associated with lymphatic invasion of cancer in vitro. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/57871 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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