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A polymorphism of the TIM-1 IgV domain: Implications for the susceptibility to filovirus infection

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Title: A polymorphism of the TIM-1 IgV domain: Implications for the susceptibility to filovirus infection
Authors: Kuroda, Makoto Browse this author
Fujikura, Daisuke Browse this author
Noyori, Osamu Browse this author
Kajihara, Masahiro Browse this author
Maruyama, Junki Browse this author
Miyamoto, Hiroko Browse this author
Yoshida, Reiko Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: Filovirus
Issue Date: 12-Dec-2014
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 455
Issue: 3-4
Start Page: 223
End Page: 228
Publisher DOI: 10.1016/j.bbrc.2014.10.144
PMID: 25449273
Abstract: Filoviruses, including Ebola and Marburg viruses, cause severe hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Human T-cell immunoglobulin and mucin domain 1 (TIM-I) is one of the host proteins that have been shown to promote filovirus entry into cells. In this study, we cloned TIM-1 genes from three different African green monkey kidney cell lines (Vero E6, COS-I, and BSC-1) and found that TIM-1 of Vero E6 had a 23-amino acid deletion and 6 amino acid substitutions compared with those of COS-1 and BSC-1. Interestingly, Vero E6 TIM-I had a greater ability to promote the infectivity of vesicular stomatitis viruses pseudotyped with filovirus glycoproteins than COS-1-derived TIM-I. We further found that the increased ability of Vero E6 TIM-1 to promote virus infectivity was most likely due to a single amino acid difference between these TIM-1s. These results suggest that a polymorphism of the TIM-I molecules is one of the factors that influence cell susceptibility to filovirus infection, providing a new insight into the molecular basis for the filovirus host range. (C) 2014 Elsevier Inc. All rights reserved.
Type: article (author version)
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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