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A polymorphism of the TIM-1 IgV domain: Implications for the susceptibility to filovirus infection
| Title: | A polymorphism of the TIM-1 IgV domain: Implications for the susceptibility to filovirus infection |
| Authors: | Kuroda, Makoto Browse this author | | Fujikura, Daisuke Browse this author | | Noyori, Osamu Browse this author | | Kajihara, Masahiro Browse this author | | Maruyama, Junki Browse this author | | Miyamoto, Hiroko Browse this author | | Yoshida, Reiko Browse this author | | Takada, Ayato Browse this author →KAKEN DB |
| Keywords: | Filovirus | | Entry | | Receptor | | TIM-1 | | Polymorphism | | Susceptibility |
| Issue Date: | 12-Dec-2014 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 455 |
| Issue: | 3-4 |
| Start Page: | 223 |
| End Page: | 228 |
| Publisher DOI: | 10.1016/j.bbrc.2014.10.144 |
| PMID: | 25449273 |
| Abstract: | Filoviruses, including Ebola and Marburg viruses, cause severe hemorrhagic fever in humans and nonhuman primates with mortality rates of up to 90%. Human T-cell immunoglobulin and mucin domain 1 (TIM-I) is one of the host proteins that have been shown to promote filovirus entry into cells. In this study, we cloned TIM-1 genes from three different African green monkey kidney cell lines (Vero E6, COS-I, and BSC-1) and found that TIM-1 of Vero E6 had a 23-amino acid deletion and 6 amino acid substitutions compared with those of COS-1 and BSC-1. Interestingly, Vero E6 TIM-I had a greater ability to promote the infectivity of vesicular stomatitis viruses pseudotyped with filovirus glycoproteins than COS-1-derived TIM-I. We further found that the increased ability of Vero E6 TIM-1 to promote virus infectivity was most likely due to a single amino acid difference between these TIM-1s. These results suggest that a polymorphism of the TIM-I molecules is one of the factors that influence cell susceptibility to filovirus infection, providing a new insight into the molecular basis for the filovirus host range. (C) 2014 Elsevier Inc. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/57926 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 高田 礼人
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