Growth Inhibition of Head and Neck Squamous Cell Carcinoma Cells by sgRNA Targeting the Cyclin D1 mRNA Based on TRUE Gene Silencing

Iizuka, Satoshi; Oridate, Nobuhiko; Nashimoto, Masayuki; Fukuda, Satoshi; Tamura, Masato

doi:10.1371/journal.pone.0114121


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Growth Inhibition of Head and Neck Squamous Cell Carcinoma Cells by sgRNA Targeting the Cyclin D1 mRNA Based on TRUE Gene Silencing

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Title:	Growth Inhibition of Head and Neck Squamous Cell Carcinoma Cells by sgRNA Targeting the Cyclin D1 mRNA Based on TRUE Gene Silencing
Authors:	Iizuka, Satoshi Browse this author
	Oridate, Nobuhiko Browse this author →KAKEN DB
	Nashimoto, Masayuki Browse this author →KAKEN DB
	Fukuda, Satoshi Browse this author →KAKEN DB
	Tamura, Masato Browse this author →KAKEN DB
Keywords:	Cisplatin
	Transfection
	Prognosis
	Head and neck cancer
	squamous cell carcinoma
	Cell proliferation
	Gene silencing
	cyclin D1
Issue Date:	1-Dec-2014
Publisher:	Public Library of Science
Journal Title:	PLOS one
Volume:	9
Issue:	12
Start Page:	e114121
Publisher DOI:	10.1371/journal.pone.0114121
PMID:	25437003
Abstract:	Head and neck squamous cell carcinoma (HNSCC) exhibits increased expression of cyclin D1 (CCND1). Previous studies have shown a correlation between poor prognosis of HNSCC and cyclin D1 overexpression. tRNase ZL-utilizing efficacious gene silencing (TRUE gene silencing) is one of the RNA-mediated gene expression control technologies that have therapeutic potential. This technology is based on a unique enzymatic property of mammalian tRNase ZL, which is that it can cleave any target RNA at any desired site by recognizing a pre-tRNA-like complex formed between the target RNA and an artificial small guide RNA (sgRNA). In this study, we designed several sgRNAs targeting human cyclin D1 mRNA to examine growth inhibition of HNSCC cells. Transfection of certain sgRNAs decreased levels of cyclin D1 mRNA and protein in HSC-2 and HSC-3 cells, and also inhibited their proliferation. The combination of these sgRNAs and cisplatin showed more than additive inhibition of cancer cell growth. These findings demonstrate that TRUE gene silencing of cyclin D1 leads to inhibition of the growth of HNSCC cells and suggest that these sgRNAs alone or combined with cisplatin may be a useful new therapy for HNSCCs.
Rights:	http://creativecommons.org/licenses/by/4.0/
Type:	article
URI:	http://hdl.handle.net/2115/57928
Appears in Collections:	歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村正人

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