HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
歯学研究院・歯学院  >
雑誌発表論文等  >

MyD88 Deficiency Alters Expression of Antimicrobial Factors in Mouse Salivary Glands


journal.pone.0113333.pdf1.62 MBPDF見る/開く

タイトル: MyD88 Deficiency Alters Expression of Antimicrobial Factors in Mouse Salivary Glands
著者: Into, Takeshi 著作を一覧する
Takigawa, Toshiya 著作を一覧する
Niida, Shumpei 著作を一覧する
Shibata, Ken-ichiro 著作を一覧する
キーワード: Immune system
Antimicrobial agents
Signal transduction
Flow cytometry
Gene expression
発行日: 2014年11月21日
出版者: Public Library of Science
誌名: PLOS one
巻: 9
号: 11
開始ページ: e113333
出版社 DOI: 10.1371/journal.pone.0113333
抄録: The surfaces of oral mucosa are protected from infections by antimicrobial proteins and natural immunoglobulins that are constantly secreted in saliva, serving as principal innate immune defense in the oral cavity. MyD88 is an important adaptor protein for signal transduction downstream of Toll-like receptors and TACI, receptors for regulation of innate immunity and B cell responses, respectively. Although MyD88-mediated signaling has a regulatory role in the intestinal mucosal immunity, its specific role in the oral cavity has remained elusive. In the present study, we assessed the influence of MyD88 deficiency on the oral innate defense, particularly the expression of antimicrobial proteins in salivary glands and production of salivary basal immunoglobulins, in mice. Microarray analysis of the whole tissues of submandibular glands revealed that the expression of several genes encoding salivary antimicrobial proteins, such as secretory leukocyte peptidase inhibitor (SLPI), S100A8, and lactotransferrin, was reduced due to MyD88 deficiency. Histologically, SLPI-expressing acinar cells were evidently decreased in the glands from MyD88 deficient mice compared to wild-type mice. Flow cytometric analysis revealed that B cell populations, including B-1 cells and IgA(+) plasma cells, residing in submandibular glands were increased by MyD88 deficiency. The level of salivary anti-phosphorylcholine IgA was elevated in MyD88 deficient mice compared to wild-type mice. Thus, this study provides a detailed description of the effect of MyD88 deficiency on expression of several salivary antimicrobial factors in mice, illustrating the role for MyD88-mediated signaling in the innate immune defense in the oral cavity.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 柴田 健一郎


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学