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Induction of Macrophage-Like Immunosuppressive Cells from Mouse ES Cells That Contribute to Prolong Allogeneic Graft Survival
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Induction of Macrophage-Like Immunosuppressive Cells from Mouse ES Cells That Contribute to Prolong Allogeneic Graft Survival |
| Authors: | Kudo, Hiroya Browse this author | | Wada, Haruka Browse this author →KAKEN DB | | Sasaki, Hajime Browse this author | | Tsuji, Hyuma Browse this author | | Otsuka, Ryo Browse this author | | Baghdadi, Muhammad Browse this author | | Kojo, Satoshi Browse this author →KAKEN DB | | Chikaraishi, Tatsuya Browse this author →KAKEN DB | | Seino, Ken-ichiro Browse this author |
| Issue Date: | 30-Oct-2014 |
| Publisher: | Public Library of Science |
| Journal Title: | PLOS one |
| Volume: | 9 |
| Issue: | 10 |
| Start Page: | e111826 |
| Publisher DOI: | 10.1371/journal.pone.0111826 |
| Abstract: | Recent progress in regenerative medicine has enabled the utilization of pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs) as a donor resource for transplantation. However, immune suppression is still needed when the donor-recipient combination is allogeneic. Protection of ESCs-derived grafts from host immune response might be achieved thought the utilization of immunosuppressive cells generated from ESCs. In the present study, we show that a certain fraction of immunosuppressive cells can be generated from ESCs and help to suppress immune response against allogeneic grafts. ESCs-derived suppressor cells (ES-SCs) resembled macrophages in terms of cell surface molecule and gene expressions. Furthermore, gene expression analysis including microarray showed that ES-SCs have M1/M2 hybrid phenotype with high expression of genes correlated to immunosuppression of T cell response. Indeed, ES-SCs were effective to block allogeneic T cell proliferation in a nitric oxide-dependent manner, and prolonged the survival of ESCs-derived embryoid bodies or cardiomyocytes grafts transplanted into mouse kidney capsule. Thus, we consider the potential use of these ESCs-derived macrophage-like immunosuppressive cells as cellular therapies to promote long-term graft survival in future therapies. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/57942 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 清野 研一郎
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