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Cytosolic chaperonin prevents polyglutamine toxicity with altering the aggregation state.
|Cytosolic chaperonin prevents polyglutamine toxicity with altering the aggregation state.
|Kitamura, Akira Browse this author →KAKEN DB
|Kubota, Hiroshi Browse this author →KAKEN DB
|Pack, Chan-Gi Browse this author
|Matsumoto, Gen Browse this author →KAKEN DB
|Hirayama, Shoshiro Browse this author
|Takahashi, Yasuo Browse this author →KAKEN DB
|Kimura, Hiroshi Browse this author
|Kinjo, Masataka Browse this author →KAKEN DB
|Morimoto, Richard Browse this author
|Nagata, Kazuhiro Browse this author →KAKEN DB
|Nature cell biology
|Polyglutamine (polyQ)-expansion proteins cause neurodegenerative disorders including Huntington's disease, Kennedy's disease and various ataxias. The cytotoxicity of these proteins is associated with the formation of aggregates or other conformationally toxic species. Here, we show that the cytosolic chaperonin CCT (also known as TRiC) can alter the course of aggregation and cytotoxicity of huntingtin (Htt)-polyQ proteins in mammalian cells. Disruption of the CCT complex by RNAi-mediated knockdown enhanced Htt-polyQ aggregate formation and cellular toxicity. Analysis of the aggregation states of the Htt-polyQ proteins by fluorescence correlation spectroscopy revealed that CCT depletion results in the appearance of soluble Htt-polyQ aggregates. Similarly, overexpression of all eight subunits of CCT suppressed Htt aggregation and neuronal cell death. These results indicate that CCT has an essential role in protecting against the cytotoxicity of polyQ proteins by affecting the course of aggregation.
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|生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
Submitter: 北村 朗