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DNA barcoding for identification of sand fly species (Diptera: Psychodidae) from leishmaniasis-endemic areas of Peru

ActaTropica v.145p.45.pdf853.35 kBPDF見る/開く

タイトル: DNA barcoding for identification of sand fly species (Diptera: Psychodidae) from leishmaniasis-endemic areas of Peru
著者: Nzelu, Chukwunonso O. 著作を一覧する
Cáceres, Abraham G. 著作を一覧する
Arrunátegui-Jiménez, Martín J. 著作を一覧する
Lañas-Rosas, Máximo F. 著作を一覧する
Yañez-Trujillano, Henrry H. 著作を一覧する
Luna-Caipo, Deysi V. 著作を一覧する
Holguín-Mauricci, Carlos E. 著作を一覧する
Katakura, Ken 著作を一覧する
Hashiguchi, Yoshihisa 著作を一覧する
Kato, Hirotomo 著作を一覧する
キーワード: DNA barcode
sand flies
発行日: 2015年 3月
出版者: Elsevier
誌名: Acta Tropica
巻: 145
開始ページ: 45
終了ページ: 51
出版社 DOI: 10.1016/j.actatropica.2015.02.003
抄録: Phlebotomine sand flies are the only proven vectors of leishmaniases, a group of human and animal diseases. Accurate knowledge of sand fly species identification is essential in understanding the epidemiology of leishmaniasis and vector control in endemic areas. Classical identification of sand fly species based on morphological characteristics often remains difficult and requires taxonomic expertise. Here, we generated DNA barcodes of the cytochrome c oxidase subunit 1 (COI) gene using 159 adult specimens morphologically identified to be 19 species of sand flies, belonging to 6 subgenera/species groups circulating in Peru, including the vector species. Neighbor-joining (NJ) analysis based on Kimura 2-Parameter genetic distances formed non-overlapping clusters for all species. The levels of intraspecific genetic divergence ranged from 0 to 5.96%, whereas interspecific genetic divergence among different species ranged from 8.39 to 19.08%. The generated COI barcodes could discriminate between all the sand fly taxa. Besides its success in separating known species, we found that DNA barcoding is useful in revealing population differentiation and cryptic diversity, and thus promises to be a valuable tool for epidemiological studies of leishmaniasis. Copyright (C) 2015 Published by Elsevier B.V. All rights reserved.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 加藤 大智


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