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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
DNA barcoding for identification of sand fly species (Diptera: Psychodidae) from leishmaniasis-endemic areas of Peru
| Title: | DNA barcoding for identification of sand fly species (Diptera: Psychodidae) from leishmaniasis-endemic areas of Peru |
| Authors: | Nzelu, Chukwunonso O. Browse this author | | Cáceres, Abraham G. Browse this author | | Arrunátegui-Jiménez, Martín J. Browse this author | | Lañas-Rosas, Máximo F. Browse this author | | Yañez-Trujillano, Henrry H. Browse this author | | Luna-Caipo, Deysi V. Browse this author | | Holguín-Mauricci, Carlos E. Browse this author | | Katakura, Ken Browse this author →KAKEN DB | | Hashiguchi, Yoshihisa Browse this author →KAKEN DB | | Kato, Hirotomo Browse this author →KAKEN DB |
| Keywords: | DNA barcode | | sand flies | | leishmaniasis | | vector | | Peru |
| Issue Date: | Mar-2015 |
| Publisher: | Elsevier |
| Journal Title: | Acta Tropica |
| Volume: | 145 |
| Start Page: | 45 |
| End Page: | 51 |
| Publisher DOI: | 10.1016/j.actatropica.2015.02.003 |
| PMID: | 25697864 |
| Abstract: | Phlebotomine sand flies are the only proven vectors of leishmaniases, a group of human and animal diseases. Accurate knowledge of sand fly species identification is essential in understanding the epidemiology of leishmaniasis and vector control in endemic areas. Classical identification of sand fly species based on morphological characteristics often remains difficult and requires taxonomic expertise. Here, we generated DNA barcodes of the cytochrome c oxidase subunit 1 (COI) gene using 159 adult specimens morphologically identified to be 19 species of sand flies, belonging to 6 subgenera/species groups circulating in Peru, including the vector species. Neighbor-joining (NJ) analysis based on Kimura 2-Parameter genetic distances formed non-overlapping clusters for all species. The levels of intraspecific genetic divergence ranged from 0 to 5.96%, whereas interspecific genetic divergence among different species ranged from 8.39 to 19.08%. The generated COI barcodes could discriminate between all the sand fly taxa. Besides its success in separating known species, we found that DNA barcoding is useful in revealing population differentiation and cryptic diversity, and thus promises to be a valuable tool for epidemiological studies of leishmaniasis. Copyright (C) 2015 Published by Elsevier B.V. All rights reserved. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/58020 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加藤 大智
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