HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
水産科学院・水産科学研究院  >
雑誌発表論文等  >

The variable 5' end of the 16S rRNA gene as a novel barcoding tool for scallops (Bivalvia, Pectinidae)

フルテキスト
Manuscript-2.pdf458.96 kBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/58028

タイトル: The variable 5' end of the 16S rRNA gene as a novel barcoding tool for scallops (Bivalvia, Pectinidae)
著者: Marin, Alan 著作を一覧する
Fujimoto, Takafumi 著作を一覧する
Arai, Katsutoshi 著作を一覧する
キーワード: Mitochondrial DNA
DNA barcoding
Scallops
Species identification
発行日: 2015年 1月
出版者: Springer
誌名: Fisheries science
巻: 81
号: 1
開始ページ: 73
終了ページ: 81
出版社 DOI: 10.1007/s12562-014-0819-6
抄録: Scallops (Bivalvia, Pectinidae) are among the most valuable source of marine food. With about 350 extant species distributed worldwide and a total global production comprising 18 species, the development of proper species-level identification assays is imperative. DNA barcoding has proven to be a useful tool in species identification. A partial region at the 5' end of the mitochondrial cytochrome c oxidase subunit I (COI) gene, known as the "Folmer region," was proposed as the most suitable DNA barcoding marker. However, Folmer primers have failed to amplify polymerase chain reaction (PCR) products in different organisms, including scallops. Searching for an alternative barcoding gene region, we analyzed the complete mitochondrial 16S rRNA gene in 15 scallop species. We found that the interspecific variation at the 5' end is twice as high as that at the 3' end. Based on that evidence, we designed a novel Pectinidae family-specific primer set, aiming to amplify a partial region at the 5' end of the 16S rRNA gene, and tested its suitability as a barcoding tool. A neighbor-joining analysis identified correctly 100 % of the scallop specimens analyzed, with high bootstrap support. Our new primers are well suited for DNA barcoding analysis and may contribute to scallop food industry surveys, as well as routine taxonomic surveys.
Rights: The final publication is available at www.springerlink.com
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/58028
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 荒井 克俊

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学