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Leader cells regulate collective cell migration via Rac activation in the downstream signaling of integrin beta 1 and PI3K

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タイトル: Leader cells regulate collective cell migration via Rac activation in the downstream signaling of integrin beta 1 and PI3K
著者: Yamaguchi, Naoya 著作を一覧する
Mizutani, Takeomi 著作を一覧する
Kawabata, Kazushige 著作を一覧する
Haga, Hisashi 著作を一覧する
発行日: 2015年 1月 7日
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 5
開始ページ: 7656
出版社 DOI: 10.1038/srep07656
抄録: Collective cell migration plays a crucial role in several biological processes, such as embryonic development, wound healing, and cancer metastasis. Here, we focused on collectively migrating Madin-Darby Canine Kidney (MDCK) epithelial cells that follow a leader cell on a collagen gel to clarify the mechanism of collective cell migration. First, we removed a leader cell from the migrating collective with a micromanipulator. This then caused disruption of the cohesive migration of cells that followed in movement, called "follower'' cells, which showed the importance of leader cells. Next, we observed localization of active Rac, integrin beta 1, and PI3K. These molecules were clearly localized in the leading edge of leader cells, but not in follower cells. Live cell imaging using active Rac and active PI3K indicators was performed to elucidate the relationship between Rac, integrin beta 1, and PI3K. Finally, we demonstrated that the inhibition of these molecules resulted in the disruption of collective migration. Our findings not only demonstrated the significance of a leader cell in collective cell migration, but also showed that Rac, integrin beta 1, and PI3K are upregulated in leader cells and drive collective cell migration.
資料タイプ: article
URI: http://hdl.handle.net/2115/58058
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 芳賀 永

 

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