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Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/58148

Title: Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation
Authors: Tzung, Keh-Weei Browse this author
Goto, Rie Browse this author
Saju, Jolly M. Browse this author
Sreenivasan, Rajini Browse this author
Saito, Taiju Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Yamaha, Etsuro Browse this author →KAKEN DB
Hossain, Mohammad Sorowar Browse this author
Calvert, Meredith E.K. Browse this author
Orbán, László Browse this author
Issue Date: 13-Jan-2015
Publisher: Elsevier
Journal Title: Stem Cell Reports
Volume: 4
Issue: 1
Start Page: 61
End Page: 73
Publisher DOI: 10.1016/j.stemcr.2014.10.011
Abstract: As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation.We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome.
Rights: http://creativecommons.org/licenses/by-nc-nd/3.0/
Type: article
URI: http://hdl.handle.net/2115/58148
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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