|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation
This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
| Title: | Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation |
| Authors: | Tzung, Keh-Weei Browse this author | | Goto, Rie Browse this author | | Saju, Jolly M. Browse this author | | Sreenivasan, Rajini Browse this author | | Saito, Taiju Browse this author | | Arai, Katsutoshi Browse this author →KAKEN DB | | Yamaha, Etsuro Browse this author →KAKEN DB | | Hossain, Mohammad Sorowar Browse this author | | Calvert, Meredith E.K. Browse this author | | Orbán, László Browse this author |
| Issue Date: | 13-Jan-2015 |
| Publisher: | Elsevier |
| Journal Title: | Stem Cell Reports |
| Volume: | 4 |
| Issue: | 1 |
| Start Page: | 61 |
| End Page: | 73 |
| Publisher DOI: | 10.1016/j.stemcr.2014.10.011 |
| Abstract: | As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation.We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome. |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/58148 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 荒井 克俊
|
