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The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

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Title: The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions
Authors: Yambao, Ma. Leonora M. Browse this author
Masuta, Chikara Browse this author →KAKEN DB
Nakahara, Kenji Browse this author →KAKEN DB
Uyeda, Ichiro4 Browse this author →KAKEN DB
Authors(alt): 上田, 一郎4
Issue Date: Oct-2003
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 84
Start Page: 2861
End Page: 2869
Publisher DOI: 10.1099/vir.0.19312-0
PMID: 13679621
Abstract: Interactions between the major proteins of Clover yellow vein virus (ClYVV) were investigated using a GAL4 transcription activator-based yeast two-hybrid system (YTHS). Self-interactions manifested by VPg and HCPro and an interaction between NIb and NIaPro were observed in ClYVV. In addition, a strong HCPro–VPg interaction was detected by both YTHS and by in vitro far-Western blot analysis in ClYVV. A potyvirus HCPro–VPg interaction has not been reported previously. Using YTHS, domains in ClYVV for the VPg self-interaction and the HCPro–VPg interaction were mapped. The VPg C-terminal region (38 amino acids) was important for the VPg–VPg interaction and the central 19 amino acids were needed for the HCPro–VPg interaction.
Type: article
URI: http://hdl.handle.net/2115/5816
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上田 一郎

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