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The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

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タイトル: The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions
著者: Yambao, Ma. Leonora M. 著作を一覧する
Masuta, Chikara 著作を一覧する
Nakahara, Kenji 著作を一覧する
Uyeda, Ichiro 著作を一覧する
発行日: 2003年10月
出版者: Society for General Microbiology
誌名: Journal of General Virology
巻: 84
開始ページ: 2861
終了ページ: 2869
出版社 DOI: 10.1099/vir.0.19312-0
抄録: Interactions between the major proteins of Clover yellow vein virus (ClYVV) were investigated using a GAL4 transcription activator-based yeast two-hybrid system (YTHS). Self-interactions manifested by VPg and HCPro and an interaction between NIb and NIaPro were observed in ClYVV. In addition, a strong HCPro–VPg interaction was detected by both YTHS and by in vitro far-Western blot analysis in ClYVV. A potyvirus HCPro–VPg interaction has not been reported previously. Using YTHS, domains in ClYVV for the VPg self-interaction and the HCPro–VPg interaction were mapped. The VPg C-terminal region (38 amino acids) was important for the VPg–VPg interaction and the central 19 amino acids were needed for the HCPro–VPg interaction.
資料タイプ: article
URI: http://hdl.handle.net/2115/5816
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 上田 一郎

 

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