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Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle.

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Title: Dynamics of intracellular phospholipid membrane organization during oocyte maturation and successful vitrification of immature oocytes retrieved by ovum pick-up in cattle.
Authors: Aono, Akira Browse this author
Nagatomo, Hiroaki Browse this author
Takuma, Tetsuya Browse this author
Nonaka, Rika Browse this author
Ono, Yoshitaka Browse this author
Wada, Yasuhiko Browse this author
Abe, Yasuyuki Browse this author
Takahashi, Masashi Browse this author
Watanabe, Tomomasa Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: Bovine immature oocytes
Vitrification
Intracellular phospholipid membrane
Issue Date: May-2013
Publisher: Elsevier
Journal Title: Theriogenology
Volume: 79
Issue: 8
Start Page: 1146
End Page: 1152.e1
Publisher DOI: 10.1016/j.theriogenology.2013.02.009
PMID: 23534997
Abstract: The objective was to determine if immature bovine oocytes with cumulus cells at the germinal vesicle (GV) stage could be vitrified by aluminum sheets (AS; pieces of sheet-like aluminum foil). Cleavage rates in fertilized oocytes previously vitrified by the AS procedure were higher than those vitrified by a nylon-mesh holder (NM) procedure (89.3 ± 2.1% vs. 65.0 ± 3.7%). Cleaved embryos derived from the AS but not from the NM procedures developed to blastocysts. Furthermore, to investigate the effects of vitrifying GV oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes, the intracellular phospholipid membrane (IM) was stained with the lipophilic fluorescent dye, 3,3'-dioctadecyloxa-carbocyanine perchlorate. After vitrification by AS, the IM remained intact relative to that of oocytes vitrified by NM. During in vitro maturation, reorganization of the IM was also undamaged in oocytes vitrified by AS before oocyte maturation, and the IM within oocytes vitrified by the NM procedure was evidently impaired. Finally, vitrification (AS) was used for GV oocytes collected using the ovum pick-up method. A bull calf was born after in vitro production and subsequent embryo transfer. The vitrification techniques described herein should facilitate generation of viable in vitro production bovine blastocysts using oocytes recovered using the ovum pick-up method.
Type: article (author version)
URI: http://hdl.handle.net/2115/58187
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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