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Hypothesis on particle structure and assembly of rice dwarf phytoreovirus: interactions among multiple structural proteins

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Title: Hypothesis on particle structure and assembly of rice dwarf phytoreovirus: interactions among multiple structural proteins
Authors: Ueda, Shigenori Browse this author
Masuta, Chikara Browse this author →KAKEN DB
Uyeda, Ichiro3 Browse this author →KAKEN DB
Authors(alt): 上田, 一郎3
Issue Date: 1997
Publisher: Society for General Microbiology
Journal Title: Journal of General Virology
Volume: 78
Start Page: 3135
End Page: 3140
Publisher DOI: 10.1099/0022-1317-78-12-3135
Abstract: To study the morphogenesis and packaging of rice dwarf phytoreovirus (RDV), the interactions among multiple structural proteins were analysed using both the yeast two-hybrid system and far-Western blotting analysis. The following protein-protein interactions were observed. P3 (major core protein) bound to itself as well as to P7 (nucleic acid-binding protein) and P8 (major outer capsid protein). P7 bound to P1 (RNA-dependent RNA polymerase) and P8, in addition to P3. Based on these findings, we hypothesize that the core shell structure is based on P3-P3 interactions and that P7 has the ability to bind to multiple structural proteins as well as to genomic RNAs during viral particle assembly. Based on the observed protein-protein interactions and on computer-aided analysis of the numbers of structural proteins per particle, possible RDV assembly events are proposed.
Type: article
URI: http://hdl.handle.net/2115/5819
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上田 一郎

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