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Hokkaido University Collection of Scholarly and Academic Papers >
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Peer-reviewed Journal Articles, etc >
Hypothesis on particle structure and assembly of rice dwarf phytoreovirus: interactions among multiple structural proteins
| Title: | Hypothesis on particle structure and assembly of rice dwarf phytoreovirus: interactions among multiple structural proteins |
| Authors: | Ueda, Shigenori Browse this author | | Masuta, Chikara Browse this author →KAKEN DB | | Uyeda, Ichiro3 Browse this author →KAKEN DB |
| Authors(alt): | 上田, 一郎3 |
| Issue Date: | 1997 |
| Publisher: | Society for General Microbiology |
| Journal Title: | Journal of General Virology |
| Volume: | 78 |
| Start Page: | 3135 |
| End Page: | 3140 |
| Publisher DOI: | 10.1099/0022-1317-78-12-3135 |
| Abstract: | To study the morphogenesis and packaging of rice dwarf phytoreovirus (RDV), the interactions among multiple structural proteins were analysed using both the yeast two-hybrid system and far-Western blotting analysis. The following protein-protein interactions were observed. P3 (major core protein) bound to itself as well as to P7 (nucleic acid-binding protein) and P8 (major outer capsid protein). P7 bound to P1 (RNA-dependent RNA polymerase) and P8, in addition to P3. Based on these findings, we hypothesize that the core shell structure is based on P3-P3 interactions and that P7 has the ability to bind to multiple structural proteins as well as to genomic RNAs during viral particle assembly. Based on the observed protein-protein interactions and on computer-aided analysis of the numbers of structural proteins per particle, possible RDV assembly events are proposed. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/5819 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 上田 一郎
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