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Molecular basis of transmembrane beta-barrel formation of staphylococcal pore-forming toxins

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Title: Molecular basis of transmembrane beta-barrel formation of staphylococcal pore-forming toxins
Authors: Yamashita, Daichi Browse this author
Sugawara, Takaki Browse this author
Takeshita, Miyu Browse this author
Kaneko, Jun Browse this author →KAKEN DB
Kamio, Yoshiyuki Browse this author →KAKEN DB
Tanaka, Isao Browse this author →KAKEN DB
Tanaka, Yoshikazu Browse this author →KAKEN DB
Yao, Min Browse this author →KAKEN DB
Issue Date: Sep-2014
Publisher: Nature Publishing Group
Journal Title: Nature Communications
Volume: 5
Start Page: 4897
Publisher DOI: 10.1038/ncomms5897
PMID: 25263813
Abstract: Pathogenic bacteria secrete pore-forming toxins (PFTs) to attack target cells. PFTs are expressed as water-soluble monomeric proteins, which oligomerize into nonlytic prepore intermediates on the target cell membrane before forming membrane-spanning pores. Despite a wealth of biochemical data, the lack of high-resolution prepore structural information has hampered understanding of the beta-barrel formation process. Here, we report crystal structures of staphylococcal gamma-haemolysin and leucocidin prepores. The structures reveal a disordered bottom half of the beta-barrel corresponding to the transmembrane region, and a rigid upper extramembrane half. Spectroscopic analysis of fluorescently labelled mutants confirmed that the prepore is distinct from the pore within the transmembrane region. Mutational analysis also indicates a pivotal role for the glycine residue located at the lipid-solvent interface as a 'joint' between the two halves of the beta-barrel. These observations suggest a two-step transmembrane beta-barrel pore formation mechanism in which the upper extramembrane and bottom transmembrane regions are formed independently.
Type: article (author version)
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田中 良和

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