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Molecular Cloning and Characterization of the Expression Profiles of Vitellogenin Transcripts in the Dojo Loach (Misgurnus anguillicaudatus) in Response to 17 alpha-ethinylestradioland 17 beta-estradiol Administration

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Title: Molecular Cloning and Characterization of the Expression Profiles of Vitellogenin Transcripts in the Dojo Loach (Misgurnus anguillicaudatus) in Response to 17 alpha-ethinylestradioland 17 beta-estradiol Administration
Authors: Wu, Meiqin Browse this author
Nishimiya, Osamu Browse this author
Nakamori, Misato Browse this author
Soyano, Kiyoshi Browse this author
Todo, Takashi Browse this author
Hara, Akihiko Browse this author
Hiramatsu, Naoshi Browse this author
Keywords: 17 alpha-ethinylestradiol
17 beta-estradiol
estrogenic endocrine-disrupting chemicals
Misgurnus anguillicaudatus
quantitative realtime PCR
vitellogenin
Issue Date: Apr-2014
Publisher: Zoological Society of Japan
Journal Title: Zoological Science
Volume: 31
Issue: 4
Start Page: 202
End Page: 212
Publisher DOI: 10.2108/zs130223
PMID: 24694222
Abstract: The gene, vitellogenin (vtg) was cloned and characterized in the dojo loach (Misgurnus anguillicaudatus), an indigenous freshwater species in East Asia, in order to develop tools for detecting the effects of estrogenic endocrine-disrupting chemicals (EEDCs). Full-length cDNAs encoding seven distinct vtg transcripts (vtg1-7) were obtained. The corresponding deduced amino acid sequences (Vtg1-7) were divided into two types; type I (Vtg1-6; 89-99% identical), which contained both lipovitellin (Lv) and phosvitin (Pv), and type II (Vtg7), which contained Lv alone. Phylogenetic analysis revealed that the type I and type II Vtgs in the loach could be classified as VtgAo1 and VtgC types, respectively. Immuno-biochemical analyses using type-specific Vtg antisera revealed that VtgAo1 proteins appeared to be the major Vtg type in this species. Males were administered (aqueous exposure) either 17 beta-estradiol (E2) or 17 alpha-ethinylestradiol (EE2), the results from which were used to determine that hepatic vtgAo1 expression was estrogen-sensitive. The precise classification of the loach vtg/Vtg products, as well as their induction profiles following the estrogenic stimulation, provide a basis for their use as sensitive biomarkers when EEDC activities are evaluated in the freshwater environments in East Asia.
Type: article
URI: http://hdl.handle.net/2115/58268
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 平松 尚志

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